Comprehensive measurement of proteinases and proteinase inhibitors within osteoarthritic knee cartilage and synovial fluid collected from osteoarthritic knee joints

综合测量骨关节炎膝关节软骨和从骨关节炎膝关节收集的滑液中的蛋白酶和蛋白酶抑制剂

• 批准号: 22791411
• 负责人: IWASAWA Mituyasu
• 金额: $ 2.58万
• 依托单位: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791411/
• 关键词: 変形性関節症; タンパク分解酵素; 関節軟骨; ウロキナーゼ; プラスミン

In order to determine the amounts of proteinases within OA cartilage, proteins were extracted from cartilage matrix, and concentrations of various proteinases were determined by ELISA and Luminex, together with those of endogenous proteinase inhibitors. Synovial fluid was collected from OA knees, and the amounts of proteinases and endogenous inhibitors were determined as well. The results of our analysis revealed that OA cartilage contains various kinds of proteinases besides MMP-13 and ADAMTS-4 and 5, which have been supposed to be primary proteinases in OA. Again, our study has revealed that the synovial fluid from OA joints contains considerable amounts of MMP-1, 2 and 3 together with TIMP-1 and 2.

为了测定 OA 软骨内蛋白酶的含量，从软骨基质中提取蛋白质，并通过 ELISA 和 Luminex 测定各种蛋白酶以及内源性蛋白酶抑制剂的浓度。从 OA 膝关节收集滑液，并测定蛋白酶和内源性抑制剂的量。我们的分析结果表明，OA软骨中除了MMP-13、ADAMTS-4和5之外还含有多种蛋白酶，这些酶被认为是OA中的主要蛋白酶。我们的研究再次表明，OA 关节的滑液含有大量的 MMP-1、2 和 3 以及 TIMP-1 和 2。

项目成果

Synovial fluid from osteoarthritic knee joints contains various proteinases and proteinase inhibitors

骨关节炎膝关节的滑液含有多种蛋白酶和蛋白酶抑制剂

• 作者: Iwasawa M;et al.
• 通讯作者: et al.

Determination of the amounts of various proteinases and endogenous inhibitors within OA and control cartilages

测定 OA 和对照软骨中各种蛋白酶和内源性抑制剂的含量

• 作者: Iwasawa M;et al.
• 通讯作者: et al.