The elucidation of the cell proliferation control mechanism and the infection mechanism to bladder mucosa of BCG via cAMP

1.cAMP激活剂对膀胱癌细胞株BCG感染率的影响：卡介苗感染人膀胱癌细胞系后，给予cAMP激活剂。结论：1.卡介苗感染控制在cAMP激活剂浓度范围内。CAMP相关细胞生长因子变化检测：与cAMP激活剂非给药组相比，与cAMP相关的细胞生长因子PKA的表达增加约2~3倍。CAMP激活剂对肿瘤细胞增殖的影响：首先，给小鼠注射BBN。8周后，将卡介苗和cAMP激活剂分别注入膀胱和腹腔。26周后，摘除膀胱，称量膀胱重量。CAMP激活剂非给药组和cAMP激活剂给药组大鼠膀胱重量分别为2.68g和2.83g。CAMP激活剂非给药组与cAMP激活剂给药组相比，膀胱重量有减轻的趋势，但差异无统计学意义。

1. Influence on BCG infection rate in bladder cancer cell line by cAMP activator administering: After BCG had been infected with the human bladder cancer cell line, the cAMP activator was administered. The infection of BCG was controlled to the density of cAMP activator.2. Examination of change in cellular growth factor related to cAMP: The expression of PKA that is the cellular growth factor related to cAMP has increased by about 2-3 times compared with cAMP activator non-administering group.3. Influence on tumor proliferation by cAMP activator administering: First, BBN was administered to the mouse. After 8 weeks, BCG and cAMP activator were administered to the bladder and ventral cavity, respectively. After 26 weeks, the bladders were removed and the bladder was weighed. The weight of the bladder was 2.68g and 2.83g in the cAMP activator non-administration group and the cAMP activator administering group respectively. A significant difference was not admitted though the tendency with a little weight of the bladder was shown in the cAMP activator non-administration group compared with the cAMP activator administering group.