Analysis of functional role of intereukin-6 secreted by tumor-associated macrophage in ovarian cancer ascites

肿瘤相关巨噬细胞分泌的白细胞介素6在卵巢癌腹水中的功能作用分析

• 批准号: 22791529
• 负责人: ISOBE Aki
• 金额: $ 2.25万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791529/
• 关键词: 卵巣癌; 腹膜播種; TAM; IL-6; tocilizumab

Ascites were collected aseptically, and CD11b positive cells(TAMs) were purified by positive selection using magnetic-activate cell sorting(MACS) technology. Co-culture of ovarian cancer cell line, SKOV3ip1 cells, with TAMs significantly enhanced the invasive activity of cancer cells in Matrigel Boyden chamber assay. This enhancement was partially blolcked by the treatment of anti-interleukin(IL)-6 receptor antibody(Tocilizumab). Since SKOV3ip1 cells did not express IL-6 in mRNA and protein level, IL-6 secreted by TAMs were considered to play a key role in ovarian cancer invasion. In vivo ovarian cancer xenografts, i. p. treatment of Tocilizumab significantly reduced peritoneal dissemination and ascites formation. Those results suggested targeting IL-6 produced by TAMs could be a promising therapy for a subset of ovarian cancer patients.

无菌收集腹水，使用磁激活细胞分选（MACS）技术通过阳性选择纯化CD 11b阳性细胞（TAM）。卵巢癌细胞系SKOV 3 ip 1细胞与TAM共培养显著增强了癌细胞在Matrigel Boyden小室测定中的侵袭活性。抗白细胞介素（IL）-6受体抗体（托珠单抗）治疗可部分阻断这种增强作用。由于SKOV 3 ip 1细胞在mRNA和蛋白水平上均不表达IL-6，因此TAMs分泌的IL-6被认为在卵巢癌侵袭中起关键作用。体内卵巢癌异种移植物，i.托珠单抗治疗显著减少腹膜播散和腹水形成。这些结果表明，靶向TAMs产生的IL-6可能是一种有希望的卵巢癌患者亚组的治疗方法。

项目成果

卵巣がん進展時の腹水中に大量に存在する骨髄由来細胞が果たす役割の解明

阐明腹水中大量存在的骨髓来源细胞在卵巢癌进展过程中所起的作用

• 发表时间: 2011
• 作者: Isobe A;Takeda T;Wakabayashi A;Tsuiji K;Li B.;et al.;河邉真也,矢澤隆志,菅野真史,宇佐美陽子,水谷哲也,今道力敬,具云峰,松村健大,宮本薫;磯部晶.澤田健二郎.大八木知史.若林敦子.馬淵誠士.森重健一郎.木村正
• 通讯作者: 磯部晶.澤田健二郎.大八木知史.若林敦子.馬淵誠士.森重健一郎.木村正

Analysis of functional role of interleukin-6 secreted by tumor-associated macrophages in ovarian cancer ascites

肿瘤相关巨噬细胞分泌的白细胞介素6在卵巢癌腹水中的功能作用分析

• 发表时间: 2011
• 作者: Isobe;A.
• 通讯作者: A.

Aldosterone stimulates the proliferation of uterine leiomyoma cells

• DOI: 10.3109/09513590903511521
• 发表时间: 2010-05-01
• 期刊: GYNECOLOGICAL ENDOCRINOLOGY
• 影响因子: 2
• 作者: Isobe, Aki;Takeda, Takashi;Kimura, Tadashi
• 通讯作者: Kimura, Tadashi

Analysis of functional role of interleukin-6 secreted by tumor-associated macrophage in ovarian cancer ascites.

肿瘤相关巨噬细胞分泌的白细胞介素6在卵巢癌腹水中的功能作用分析。

• 发表时间: 2010
• 作者: Isobe A;Sawada K;Ohyagi C;Mabuchi S;Kimura T.
• 通讯作者: Kimura T.