Identification of myeloid derived cells which regulates ovarian cancer dissemination

从卵巢癌腹水中，我们发现CD11b+CD14+细胞与其他细胞群相比表达了显著高水平的IL-6， FACS分析显示约88%的CD11b+CD14+是甘露糖受体，CD206阳性，表明这些细胞群主要是m2极化巨噬细胞，tam。当CD11b+CD14+细胞与卵巢癌细胞共培养时，促进了癌细胞的侵袭和增殖，而抗il - 6r抗体预处理几乎完全抑制了癌细胞的侵袭和增殖，而抗il - 6r抗体不影响没有CD11b+CD14+细胞的卵巢癌细胞的侵袭和增殖，说明tam通过分泌一定量的IL-6来增强卵巢癌的增殖或侵袭。卵巢癌腹水中存在的m2极化巨噬细胞通过分泌IL-6在卵巢癌进展中发挥重要作用。靶向IL-6/IL-6R信号具有治疗卵巢癌的潜力。

From ovarian cancer ascites, we found CD11b+CD14+ cells expressed significantly high levels of IL-6 compared with other populations of cells and FACS analyses revealed about 88% of CD11b+CD14+ were mannose receptor, CD206 positive, indicating these populations of cells are predominantly M2-polarized macrophages, TAMs. When CD11b+CD14+ cells were co-cultured with ovarian cancer cells, the invasion as well as the proliferation of cancer cells were so promoted and these promotions were almost completely inhibited by the pretreatment with anti-IL-6R antibody, while anti-IL-6R antibody did not affect ovarian cancer cell invasion and proliferation without CD11b+CD14+ cells, indicating TAMs enhance ovarian cancer proliferation or invasion by secreting a certain amount of IL-6.M2-polarized macrophages present in ovarian cancer ascites play a crucial role in ovarian cancer progression by secreting IL-6. Targeting IL-6/IL-6R signaling has a therapeutic potential for ovarian cancer treatment.