Identification of myeloid derived cells which regulates ovarian cancer dissemination

鉴定调节卵巢癌传播的骨髓来源细胞

• 批准号: 24791702
• 负责人: ISOBE Aki
• 金额: $ 2.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791702/
• 关键词: 骨髄由来細胞; M2マクロファージ; IL-6; 腹膜播種; M2ﾏｸﾛﾌｧｰｼﾞ; IL-６; 卵巣癌腹膜播種

From ovarian cancer ascites, we found CD11b+CD14+ cells expressed significantly high levels of IL-6 compared with other populations of cells and FACS analyses revealed about 88% of CD11b+CD14+ were mannose receptor, CD206 positive, indicating these populations of cells are predominantly M2-polarized macrophages, TAMs. When CD11b+CD14+ cells were co-cultured with ovarian cancer cells, the invasion as well as the proliferation of cancer cells were so promoted and these promotions were almost completely inhibited by the pretreatment with anti-IL-6R antibody, while anti-IL-6R antibody did not affect ovarian cancer cell invasion and proliferation without CD11b+CD14+ cells, indicating TAMs enhance ovarian cancer proliferation or invasion by secreting a certain amount of IL-6.M2-polarized macrophages present in ovarian cancer ascites play a crucial role in ovarian cancer progression by secreting IL-6. Targeting IL-6/IL-6R signaling has a therapeutic potential for ovarian cancer treatment.

从卵巢癌腹水中，我们发现CD 11b + CD 14+细胞与其他细胞群体相比表达显著高水平的IL-6，并且FACS分析显示约88%的CD 11b + CD 14+细胞是甘露糖受体CD 206阳性的，表明这些细胞群体主要是M2极化的巨噬细胞，TAM。当CD 11b + CD 14+细胞与卵巢癌细胞共培养时，癌细胞的侵袭和增殖被促进，并且这些促进作用几乎被抗IL-6 R抗体预处理完全抑制，而抗IL-6 R抗体不影响无CD 11b + CD 14+细胞的卵巢癌细胞的侵袭和增殖，表明TAM通过分泌一定量的IL-6促进卵巢癌的增殖或侵袭。卵巢癌腹水中存在的M2极化的巨噬细胞通过分泌IL-6在卵巢癌的进展中起关键作用。靶向IL-6/IL-6 R信号传导对卵巢癌治疗具有治疗潜力。

项目成果

卵巣癌進展を制御する腹水骨髄由来細胞の同定とその役割の解析

控制卵巢癌进展的腹水骨髓来源细胞的鉴定及其作用分析

• 发表时间: 2013
• 作者: 松村謙臣;山口建;万代昌紀;小西郁生;磯部 晶
• 通讯作者: 磯部 晶

上皮性卵巣癌におけるmTOR complex 2 (mTORC2) の発現とその臨床的意義の検討

mTOR复合物2（mTORC2）在上皮性卵巢癌中的表达及其临床意义

• 发表时间: 2012
• 作者: 久松武志;馬淵誠士;河野まひる;橋本香映;磯部晶;澤田健二郎;木村正
• 通讯作者: 木村正

卵巣癌組織におけるインターロイキン 6 (IL-6) とその受容体の発現が予後に与える影響の解析

白细胞介素6(IL-6)及其受体表达对卵巢癌组织预后的影响分析

• 发表时间: 2013
• 作者: 松村謙臣;山口建;万代昌紀;小西郁生;磯部 晶;磯部 晶
• 通讯作者: 磯部 晶