Study for the mechanism of LH and FSH synthesis and secrition

促性腺激素在促性腺激素释放激素的调节下合成和分泌。GnRH脉冲频率决定特定的促性腺激素亚单位基因的表达。在垂体促性腺激素细胞中，GnRH诱导细胞外信号调节激酶(ERK)去磷酸化酶MAPK磷酸酶1(MKP1)的表达。MKP-1是在高频GnRH脉冲后特异表达的，这一效应可能参与了促性腺激素亚单位表达的差异调节。除了下丘脑促性腺激素释放激素外，垂体腺苷环化酶激活多肽(PACAP)也调节促性腺激素基因的表达。高频PACAP脉冲优先增加LHB，而低频PACAP脉冲特别增加FSHB。提示PACAP参与了促性腺激素亚单位基因表达的差异性调控。

Gonadotropins are synthesized and secreted, which is under GnRH regulation. GnRH pulse frequency determines specific gonadotropin subunit gene expression. In pituitary gonadotrophs, GnRH induces expression of the Extracellular signal-regulated kinase(ERK) dephosphorylating enzyme, MAP kinase phosphatase 1 (MKP1). MKP-1 is specifically expressed following high-frequency GnRH pulses and that this effect may participate in the differential regulation of gonadotropin subunit expression. In addition to hypothalamic GnRH, pituitary adenylate cyclase - activating polypeptide (PACAP) also regulates gonadotropin gene expression. High-frequency PACAP pulse preferentially increased Lhb, whereas low-frequency PACAP pulses specifically increased Fshb. It suggest that PACAP is involved in the differential regulation of gonadotropin subunit gene expression.