Molecular network analysis of salivary duct carcinoma for the control of distant metastasis

唾液管癌控制远处转移的分子网络分析

• 批准号: 22791570
• 负责人: SASAKI Keita
• 金额: $ 2.58万
• 依托单位: Chiba Cancer Center (Research Institute)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791570/
• 关键词: 唾液腺導管癌; microRNA; マイクロアレイ; マイクロRNA; 唾液腺悪性腫瘍; 癌抑制遺伝子; 機能性RNA

From the results of microRNA array(four pairs of the salivary duct carcinoma and normal salivary gland region), expressions of miR-21 and miR-23-24-27 cluster which have been reported as oncomiRs were increased, whereas expressions of miR-375, miR-1, and miR-200 family which have been reported as tumor suppressive miRNAs were decreased. In order to analyze the gene(s) regulated by miR-200 family, we performed in silico analysis with miRNA target search program and pathway analysis. The results suggested that genes regulated by miR-200 family are involved in cell adhesion. Furthermore, collated focal adhesion-related genes with the microarray analysis of clinical samples, over-expressed genes in cell adhesion may be involved in distant metastasis that is the clinical features of salivary duct carcinoma. These results suggested that the over-expressed genes in cell adhesion might be potential targets of novel treatment for improving the poor prognosis.

从microRNA阵列（涎腺导管癌和正常涎腺区域的四对）的结果来看，已被报道为癌miRs的miR-21和miR-23-24-27簇的表达增加，而已被报道为肿瘤抑制miRNAs的miR-375、miR-1和miR-200家族的表达减少。为了分析miR-200家族调控的基因，我们使用miRNA靶标搜索程序和途径分析进行了计算机分析。提示miR-200家族调控的基因参与了细胞粘附。此外，通过对临床标本中粘着斑相关基因的芯片分析，发现细胞粘着斑中的过表达基因可能参与了涎腺导管癌的临床特征--远处转移。这些结果表明，细胞粘附中的过表达基因可能是改善预后不良的新治疗的潜在靶点。