Molecular network analysis of salivary duct carcinoma for the control of distant metastasis

从microRNA阵列(4对涎腺导管癌和正常涎腺区)的结果来看，已被报道为癌基因的miR-21和miR-23-24-27簇的表达增加，而已报道的肿瘤抑制基因miR-375、miR-1和miR-200家族的表达降低。为了分析受miR-200家族调控的基因(S)，我们利用miRNA靶标搜索程序和通径分析进行了电子计算机分析。结果提示，miR-200家族调控的基因参与了细胞黏附。此外，结合临床标本的基因芯片分析，细胞黏附相关基因的过度表达可能与涎腺导管癌的临床特征--远处转移有关。提示高表达的细胞黏附基因可能是改善预后不良的新的治疗靶点。

From the results of microRNA array(four pairs of the salivary duct carcinoma and normal salivary gland region), expressions of miR-21 and miR-23-24-27 cluster which have been reported as oncomiRs were increased, whereas expressions of miR-375, miR-1, and miR-200 family which have been reported as tumor suppressive miRNAs were decreased. In order to analyze the gene(s) regulated by miR-200 family, we performed in silico analysis with miRNA target search program and pathway analysis. The results suggested that genes regulated by miR-200 family are involved in cell adhesion. Furthermore, collated focal adhesion-related genes with the microarray analysis of clinical samples, over-expressed genes in cell adhesion may be involved in distant metastasis that is the clinical features of salivary duct carcinoma. These results suggested that the over-expressed genes in cell adhesion might be potential targets of novel treatment for improving the poor prognosis.