Investigation of influence of Atrial fibrillation to anastomotic thrombus.

房颤对吻合口血栓影响的调查。

• 批准号: 22791732
• 负责人: SAKISAKA Masanobu
• 金额: $ 1.33万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791732/
• 关键词: 心房細動; アコニチン; 有茎皮弁; 遊離皮弁

Systemic circulation affects circulation condition of flaps and Arrhythmia is supposed to be a great risk factor during flap surgery, however, little is known about its relation. In order to investigate the influences of arrhythmia on flaps, we developed aconitine-induced atrial fibrillation model in rats without artificial respirator.Acconitine was injected into the tip of right atrial appendage trough pleural membrane. The intra-arterial blood pressure monitored with femoral artery cannulation. Yariable pulse wave was appeared with Af, but blood pressure was stable.Using this Af model without ventricular arrhythmia, we investigate influences of Af on two representative properties of flap surgeries. Survival area of pedicled flap, and survival rate of free flap.For comparison of survival area of pedicled flap with or without Af, traditionally used rat ventral Superficial Inferior Epigastric(SIE) flap(2×13cm) was used. Survival area of flap was measured 7 day after the elevation of flap. For comparison of survival rate of free flap with or without Af, smaller sized SIE flap(2×2cm) was employed. Flap survival was confirmed 7 days after operation.Average survival area of pedicled flap in Af animals(58.1±9.7%) was lower than that in controls animals(72.6±12.3%) significantly(p<0.01, Student's t-test). Average survival rate of free flap in Af animals(40%) was lower than that in controls animals(80%)(p=0.07, chi-square test).

体循环影响皮瓣的循环状态，心律失常被认为是皮瓣术中的一个重要危险因素，但其关系却鲜为人知。为了探讨心律失常对瓣膜的影响，我们建立了乌头碱诱发的大鼠心房颤动模型。股动脉插管监测动脉内压。利用无室性心律失常的房颤模型，研究房颤对瓣膜手术的两个典型特性的影响。为比较带Af与不带Af的带蒂皮瓣的成活面积，采用传统的大鼠腹侧上腹部浅下腹壁(SIE)皮瓣(2×13 cm)。在皮片抬高后第7天测量皮瓣存活面积。采用较小尺寸的SIE皮瓣(2×2 cm)，比较带Af与不带Af的游离皮瓣的成活率。Af组动物的平均成活面积(58.1±9.7%)明显低于对照组(72.6±12.3%)(p&lt；0.01，t检验)。Af组游离皮瓣平均存活率(40%)低于对照组(80%)(P=0.07，卡方检验)。

项目成果

ラット誘発心房細動モデルの開発

大鼠心房颤动模型的建立

• 发表时间: 2010
• 作者: 素輪善弘;井村徹也;沼尻敏明;西野健一;伏木信次;匂坂正信
• 通讯作者: 匂坂正信