Investigation of biofilm-related molecules in Porphyromonas gingivalis

本研究发现牙龈卟啉单胞菌血凝素HagB被糖基化并参与该微生物的生物膜形成。我们还发现，从牙龈假单胞菌培养上清中分离的外膜囊泡（omv）不仅含有牙龈蛋白酶Rgp和Kgp等多种毒力因子，还含有主要和次要的毛蛋白FimA和MfaI。我们进一步证明，omv导致口腔上皮细胞脱离细胞培养板，这是牙龈素依赖的。综上所述，我们认为HagB和omv可能分别在牙周病发展过程中与牙周组织的生物膜形成和破坏有关。

In the present study, Porphyromonas gingivalis hemagglutinin HagB was found to be glycosylated and involved in biofilm formation of this microorganism. We also found that outer membrane vesicles (OMVs) isolated from the culture supernatant of P. gingivalis contained not only several virulence factors such as gingipains including Rgp and Kgp, but also the major and minor fimbrial proteins such as FimA and MfaI. We further demonstrated that the OMVs caused detachment of oral epithelial cells from the cell culture plate, which is gingipain-dependent. Taken together, we suggest a possible role of HagB and OMVs in biofilm formation and destruction of periodontal tissue, respectively, during development of periodontal diseases.