Search of evidence on the effect with dental resin curing light irradiation against dental pulp

牙科树脂固化光已被用于树脂基复合填充物的牙齿修复和牙齿漂白的治疗。然而，对牙髓或口腔黏膜等口腔组织的影响却没有被较少考虑。本研究的目的是探讨牙树脂固化光照射对牙髓的影响。我们发现，树脂固化蓝光照射增加了离体大鼠主动脉脂质过氧化水平，用于测量丙二醛。此外，它还能呈时间依赖性地降低人主动脉血管平滑肌细胞（VSMC）的增殖活性并诱导其凋亡。综上所述，蓝光照射可在VSMC中产生过氧化氢和羟基自由基等活性氧，并诱导氧化应激相关的细胞毒性，增加脂质过氧化和细胞凋亡。此外，n -乙酰- l-半胱氨酸是典型的细胞内抗氧化剂，可防止VSMC发生与氧化应激相关的细胞毒性。

Dental resin curing light has been used to restore of tooth with resin-based composite fillings and to treat of tooth bleaching. However, it has not been less considered the effect on the oral tissues such as dental pulp or oral mucosa. The aim of this study was to investigate whether dental resin curing light irradiation is affected on the dental pulp. We found that irradiation of resin curing blue light was increased the level of lipid peroxidation on isolated rat aorta blood vessels to use the measurement of malondialdehyde. Furthermore, it was decreased the cell proliferation activity in time-dependent manner and was induced the apoptosis of human aorta vascular smooth muscle cells(VSMC). Therefore, these results indicated that reactive oxygen species such as hydrogen peroxide and hydroxyl radical were generated in VSMC by blue light irradiation, and they were induced the cytotoxicity associated with oxidative stress which was increased the lipid peroxidation and apoptosis. In addition, N-acetyl-L-cysteine which is typical intracellular antioxidants prevented VSMC from cytotoxicity associated with oxidative stress.