Search of evidence on the effect with dental resin curing light irradiation against dental pulp

牙科树脂固化光照射对牙髓影响的证据检索

• 批准号: 22791848
• 负责人: YOSHINO Fumihiko
• 金额: $ 2.58万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791848/
• 关键词: 光照射器; 活性酸素; 青色光; 酸化ストレス; アポトーシス; レジン光照射器; 活性酸素種; 抗酸化剤; ハロゲン光; 歯科用可視光照射器; 血管収縮; ノルアドレナリン; 一重項酸素; ヒドロキシルラジカル; スーパーオキシド

Dental resin curing light has been used to restore of tooth with resin-based composite fillings and to treat of tooth bleaching. However, it has not been less considered the effect on the oral tissues such as dental pulp or oral mucosa. The aim of this study was to investigate whether dental resin curing light irradiation is affected on the dental pulp. We found that irradiation of resin curing blue light was increased the level of lipid peroxidation on isolated rat aorta blood vessels to use the measurement of malondialdehyde. Furthermore, it was decreased the cell proliferation activity in time-dependent manner and was induced the apoptosis of human aorta vascular smooth muscle cells(VSMC). Therefore, these results indicated that reactive oxygen species such as hydrogen peroxide and hydroxyl radical were generated in VSMC by blue light irradiation, and they were induced the cytotoxicity associated with oxidative stress which was increased the lipid peroxidation and apoptosis. In addition, N-acetyl-L-cysteine which is typical intracellular antioxidants prevented VSMC from cytotoxicity associated with oxidative stress.

牙科树脂固化光已用于修复树脂基复合填充物的牙齿以及治疗牙齿漂白。然而，人们并没有忽视对牙髓或口腔粘膜等口腔组织的影响。本研究的目的是探讨牙科树脂固化光照射是否对牙髓产生影响。我们发现，树脂固化蓝光的照射增加了离体大鼠主动脉血管的脂质过氧化水平，以使用丙二醛的测量。此外，它还以时间依赖性方式降低细胞增殖活性，并诱导人主动脉血管平滑肌细胞（VSMC）凋亡。因此，这些结果表明蓝光照射在VSMC中产生过氧化氢和羟​​自由基等活性氧，并诱导与氧化应激相关的细胞毒性，从而增加脂质过氧化和细胞凋亡。此外，N-乙酰基-L-半胱氨酸是典型的细胞内抗氧化剂，可防止 VSMC 免受与氧化应激相关的细胞毒性。

项目成果

Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy

• DOI: 10.1254/jphs.10321fp
• 发表时间: 2011-05-01
• 期刊: JOURNAL OF PHARMACOLOGICAL SCIENCES
• 影响因子: 3.5
• 作者: Kobayashi, Kyo;Maehata, Yojiro;Lee, Masaichi-Chang-Il
• 通讯作者: Lee, Masaichi-Chang-Il

Crocetin reduces the oxidative stress induced reactive oxygen species in the SHRSPs brain

藏红花酸可减少 SHRSP 大脑中氧化应激诱导的活性氧

• 发表时间: 2011
• 期刊: Journal of Clinical Biochemistry and Nutrition
• 影响因子: 2.4
• 作者: Yoshino F.;Yoshida A.;Umigai N.;Kubo K.;Lee M-C.
• 通讯作者: Lee M-C.

Reactive oxygen species (ROS) reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells

• DOI: 10.3109/10715762.2010.490836
• 发表时间: 2010-08-01
• 期刊: FREE RADICAL RESEARCH
• 影响因子: 3.3
• 作者: Maehata, Yojiro;Ozawa, Shigeyuki;Lee, Masaichi-Chang-Il
• 通讯作者: Lee, Masaichi-Chang-Il

新規コラーゲンペプチドは紫外線照射によって引き起こされる細胞障害を抑制する

新型胶原蛋白肽可抑制紫外线照射引起的细胞损伤

• 发表时间: 2011
• 作者: 小林杏;前畑洋次郎;宮本千央;徳富文彬;杉山秀太;吉田彩佳;吉野文彦;高橋聡子;前谷崇志;川村陽介;辻村傑;岡田永三;岡田康江;西村成文;小松知子;高橋俊介;李昌一
• 通讯作者: 李昌一

Assessment of Anti-Aging Effects of Fish Products Peptide in Middle-Aged Subjects

鱼制品肽对中年受试者的抗衰老作用评估

• 发表时间: 2011
• 期刊: Anti-Aging Medicine
• 作者: Yoshino F.;Yoshida A.;Sugiyama S.;Tokutomi F.;Miyamoto C.;Maehata Y.;Kobayashi K.;Wada-Takahashi S.;Maetani T.;Okada E.;Okada Y.;Komatsu T.;Takahashi S-S.;Wan J.;Lee M-C.
• 通讯作者: Lee M-C.