Development of the novel nerve regeneration therapy for the peripheral neuropathy using microRNA

我们着眼于神经干细胞在中枢神经系统中的作用，利用DicerCKO(DicerCKO)条件基因敲除小鼠(DicerCKO)开发治疗周围神经病的新型神经再生疗法。我们已经确定了一组Dice缺陷的NSCs，它们可以自我更新，但表现出低增殖。此外，当有丝分裂原被取消时，Dester缺陷的NSCs表现出异常分化并经历细胞死亡。正如质谱学蛋白质组学方法所揭示的那样，DICER缺失会影响许多蛋白质的水平。我们发现，抗存活和/或促凋亡蛋白的增加和/或促存活和/或抗凋亡蛋白的减少是DICER缺陷NSCs细胞死亡的原因，这意味着DICER在保护细胞免受凋亡方面起着普遍的作用。

We have focused on the functions of neural stem cell in central nervous system to develop the novel nerve regeneration therapy for the peripheral neuropathy using Dicer conditional knockout mouse(DicerCKO). We have identified a population of Dicer-deficient NSCs that can self-renew, but show low proliferation. Moreover, Dicer-deficient NSCs display abnormal differentiation and undergo cell death when mitogens are withdrawn. Dicer deletion affects the levels of many proteins, as revealed by a mass spectrometry proteomic approach. We have found that an increase of anti-survival and/or pro-apoptosis proteins and a decrease of pro-survival and/or anti-apoptosis proteins contribute to the cell death of Dicer-deficient NSCs, implyinga general role for Dicer in protecting cells from apoptosis.