Induced pluripotent stem(iPS) cells in co-culture with periodontal ligament cells

本研究的目的是通过诱导多能干细胞与牙周韧带细胞共培养诱导牙周韧带干细胞。在大鼠牙周开孔模型中，移植诱导多能干细胞不仅能促进新骨的形成，还能促进脱落牙本质表面牙骨质的形成。在共培养体系中，未分化的iPS细胞减少。我们使用PrimerArray^<(R)>来研究基因表达。与对照组相比，分化后的iPS细胞BMP-2、TGF-β2、Noggin mRNA表达水平升高，Smad9、BMPR-1b mRNA表达水平降低。需要进一步的研究。

The purpose of this study was to induce the periodontal ligament stem cells from iPS cell by co-culture with periodontal ligament cells.In rat periodontal fenestration model, transplantation of the iPS cells encouraged not only new bone formation but also cementum formation on the denuded dentin surface. In the co-culture system, undifferentiated iPS cells were decreased. We investigated the gene expression using PrimerArray^<(R)>. The differentiated iPS cells increased BMP-2, TGF-β2, and Noggin mRNA expression levels compared with control, while Smad9 and BMPR-1b mRNA expression level were decreased. Further studies are necessary.