Analyses of segregation of chloroplast nucleoids

本研究通过LC-MS/MS分析鉴定了41个叶绿体类核相关蛋白。然后对两个高含量的未知功能蛋白cpNUC 1和cpNUC 3进行了分析。结果表明：（1）cpNUC 1蛋白在体外具有压缩DNA的能力，但不参与cpDNA的分离;（2）cpNUC 3蛋白参与rrn 23 RNA的隐性断裂，但不参与cpDNA的分离。另外，本文还揭示了叶绿体DNA的复制受氧化还原的调控，而不是受叶绿体分裂/细胞周期的调控

In this study, 41 proteins were identified as chloroplast nucleoid-associated proteins by LC-MS/MS analyses. And then, I analyzed about two high content unknown function proteins, cpNUC1 and cpNUC3. I found that (1) cpNUC1 protein has an ability of DNA compaction in vitro but is uninvolved in the cpDNA segregation, and that (2) cpNUC3 is involved in hidden break of rrn23 RNA but is uninvolved in the cpDNA segregation. In addition, I revealed that chloroplast DNA replication is regulated by the redox rather than chloroplast division/cell cycle