Molecular analysis for evaluation of genetic diversity and taxonomic relationships among populations of Japanese newts

我们利用线粒体DNA(NADH6，tRNAglu，cyt b)1407个核苷酸序列和核DNA(RAG-1)1208个核苷酸序列，研究了日本大陆火腹螈的系统发育关系并估计了其历史人口学特征。基于线粒体DNA的系统发育树揭示了该物种的四个主要分支(北部、中部、西部和南部分支)。除了北部支系外，核基因没有表现出明显的地理结构模式，这表明这是不完全的谱系分类结果。我们利用下一代测序仪开发了15个新的微卫星标记。利用这些标记在边界区域进行的遗传调查显示，中央支系的等位基因频率与西部支系的不同，这表明这些支系在生殖上彼此隔离。

We investigated the phylogenetic relationships and estimated the historical demography of the Japanese fire-bellied newt, Cynops pyrrhogaster, from Japanese mainlands using 1407-bp sequences of the mitochondrial DNA (NADH6, tRNAglu, cyt b) and 1208-bp sequences of nuclear DNA (Rag-1) genes. Phylogenetic trees based on mitochondrial DNA revealed four major clades (NORTHERN, CENTRAL, WESTERN, and SOUTHERN clades) within this species. Nuclear gene presented no obvious patterns of geographic structure except for the NORTHERN clade, suggesting results of incomplete lineage sorting. We developed 15 novel microsatellite markers for C. pyrrhogaster using next generation sequencer. Genetic survey using these markers around a boundary region revealed allele frequencies of CENTRAL clade differ from those of WESTERN clade, suggesting these clades reproductively isolated from each other.