Development of safe non-uniform sampling processing method and its application into proteins

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The aim of this study is to establish an integrated tool for non-uniform sampling(NUS) in multidimensional NMR for challenging the structures of proteins that are difficult to be determined with conventional NMR experiments. In this study, there were two advances.(1) We clarified that the distorted baselines in NUS spectra are mainly caused by strong peaks. By combining NUS and experiments that do not yield diagonal peaks in NOESY experiments, we could safely apply NUS method into NOESY data.(2) By modifying CYANA to handle with the chemical shifts in sparse sampling experiments, we could determine the structure of proteins without reconstructing the sparsely sampled data into 3D format.