Study of UV-light applied to macromolecular crystallography

紫外光应用于高分子晶体学的研究

• 批准号: 22770161
• 负责人: CHAVAS Leonard
• 金额: $ 2.5万
• 依托单位: High Energy Accelerator Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22770161/
• 关键词: UV照射; UV光; 高分子結晶学; フェージング

The goal of this study is to take advantage of UV-radiation for structure determination of biological macromolecule crystals. The concrete effects of UV-radiation on protein models were investigated, the aim being to develop a general methodology for UV-radiation induced phasing(UV-RIP). Exposing the crystals to an intense UV-light caused significant damages, resulting in poorer electron densities. A general methodology for UV-RIP and a semi-automated data analysis software were implemented for taking full advantage of the specific damages observed in irradiated crystals for structure determination.

本研究的目的是利用紫外光来测定生物大分子晶体的结构。研究了紫外光对蛋白质模型的具体影响，目的是建立一种通用的紫外光诱导相变(UV-RIP)方法。将晶体暴露在强烈的紫外线下会造成严重的损害，导致较低的电子密度。为了充分利用辐照晶体中观察到的特定损伤来确定结构，实现了一种通用的UV-RIP方法和一个半自动数据分析软件。

项目成果

UV-based automated crystal centering

基于紫外线的自动晶体定心

• 发表时间: 2010
• 作者: 古谷祐詞;Victor A. Lorenz-Fonfria;神取秀樹;Chavas Leonard;Yuji Furutani(Invited);Chavas Leonard;Chavas Leonard;Chavas LMG;Chavas L. M. G;Chavas L. M. G;Chavas L. M. G;Chavas LMG;Chavas LMG;Chavas L. M. G;Chavas L. M. G;Chavas L. M. G;Chavas L. M. G
• 通讯作者: Chavas L. M. G

2011 implementations and results at AR-NW12A

2011 年 AR-NW12A 的实施和结果

• 发表时间: 2012
• 作者: Chavas LMG;et al.;Chavas Leonard;古谷祐詞;古谷祐詞;古谷祐詞;古谷祐詞;Chavas Leonard;古谷祐詞;Chavas Leonard;古谷祐詞;Chavas L. M. G;Chavas L. M. G
• 通讯作者: Chavas L. M. G

UV-radiation damage phasing at PF

PF 处的紫外线辐射损伤定相

• 发表时间: 2012
• 作者: Chavas LMG;et al.;Chavas Leonard;古谷祐詞;古谷祐詞;古谷祐詞;古谷祐詞;Chavas Leonard;古谷祐詞;Chavas Leonard
• 通讯作者: Chavas Leonard

Present status of the structural biology at the Photon Factory

光子工厂结构生物学的现状

• 发表时间: 2010
• 作者: 古谷祐詞;Victor A. Lorenz-Fonfria;神取秀樹;Chavas Leonard;Yuji Furutani(Invited);Chavas Leonard;Chavas Leonard;Chavas LMG;Chavas L. M. G;Chavas L. M. G;Chavas L. M. G;Chavas LMG;Chavas LMG
• 通讯作者: Chavas LMG

Influenza virus sialidases' targeted drugs : their interaction with human sialidases

流感病毒唾液酸酶的靶向药物：它们与人类唾液酸酶的相互作用

• 发表时间: 2011
• 作者: 古谷祐詞;Victor A. Lorenz-Fonfria;神取秀樹;Chavas Leonard
• 通讯作者: Chavas Leonard