Study of GMF-based control of actin-meshwork organization during cell migration and endocytosis

细胞迁移和内吞过程中基于 GMF 的肌动蛋白网状组织控制研究

• 批准号: 22770184
• 负责人: NAKANO Kentaro
• 金额: $ 2.75万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22770184/
• 关键词: 細胞骨格; アクチン; エンドサイトーシス; 分裂酵母; メンブレントラフィック; Rho; Rab; 細胞性粘菌

Arp2/3 induces formation of actin meshwork which is required for membrane deformation in various type of cell movements. It has well been studied the molecular machinery activating Arp2/3 function. However, there is almost no study how Arp2/3-activity is down regulated during recycling of the actin cytoskeleton. Therefore, I decided to study the molecular function of a yeast homologue of the glia maturation factor GMF. Moreover, I tried to investigate cellular function of GmfA by using cellular mold which shows dynamic cell behavior.

Arp 2/3诱导肌动蛋白网络的形成，这是在各种类型的细胞运动中膜变形所必需的。Arp 2/3功能的分子机制已被研究得很好。然而，几乎没有研究Arp 2/3-活性是如何在肌动蛋白细胞骨架的再循环过程中下调的。因此，我决定研究神经胶质成熟因子GMF的酵母同源物的分子功能。此外，我试图通过使用显示动态细胞行为的细胞模型来研究GmfA的细胞功能。

项目成果

(2011) Pob1 ensures cylindrical cell shape by coupling two distinct Rho signaling events during secretory vesicle targeting

(2011) Pob1 通过在分泌囊泡靶向过程中耦合两个不同的 Rho 信号事件来确保圆柱形细胞形状

• 发表时间: 2011
• 期刊: Traffic
• 影响因子: 4.5
• 作者: Kentaro Nakano;Mika Toya;Aki Yoneda;Yukiko Asami;Akira Yamashita;Naomi Kamasaki;Masako Osumi and Masayuki Yamamoto
• 通讯作者: Masako Osumi and Masayuki Yamamoto

Dividing the spoils of growth and the cell cycle: The fission yeast as a model for the study of cytokinesis.

• DOI: 10.1002/cm.20500
• 发表时间: 2011-02
• 期刊: CYTOSKELETON
• 影响因子: 2.9
• 作者: Goya, Anupama;Takaine, Masak;Simanis, Viesturs;Nakano, Kentaro
• 通讯作者: Nakano, Kentaro

GMF is an Evolutionarily Developed ADF/Cofilin-Super Family Protein Involved in the Arp2/3 Complex-Mediated Organization of the Actin Cytoskeleton

• DOI: 10.1002/cm.20451
• 发表时间: 2010-06-01
• 期刊: CYTOSKELETON
• 影响因子: 2.9
• 作者: Nakano, Kentaro;Kuwayama, Hidekazu;Takaine, Masak
• 通讯作者: Takaine, Masak