Shed light on the flavonoid accumulation of fruit

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In order to characterize DkDHD/SDH, DkSCPL1 and DkSCPL2, which is supposed to be involved in proanthocyanidin accumulation in persimmon fruit, we tried transformation of persimmon leaf with these genes using Agrobacterium tumefaciens, and also production of DkDHD/SDH with Escherichia coli BL21(DE3) for complementation assay. We could not obtain clear results for DkSCPLs. As for DkDHD/SDH, transformation by antisense DkDHD/SDH reduce the production of proanthocyanidin, and complementation of BL21 delta aroE::KanR (DE3) with pET3a-DkDHDSDH shows this protein works as SDH in shikimic pathway.Secondly, we applied ABA and IAA to red grape cultivars around varaison to clear the effect of these plant hormones at anthocyanin accumulation of berry skin. The results indicated that the ABA treatment induces the accumulation of blue-purple anthocyanins via MybA transcription factor, while the IAA treatment reduces it.