An Exhaustive Screening and Functional Enhancement of a Useful Genes Related to Hydrogen Production by Using a Genetic Engineering Approach.

本研究的目的是提高细菌产氢量。在本次研究中，利用大肠杆菌突变体文库进行了详尽的筛选，找到了一个与产氢有关的有用基因，这有助于全面了解大肠杆菌的生物产氢；因此，有几个未表征的基因，从未报道过它们与产氢有关。此外，还研究了大肠杆菌加氢酶在甘油制氢发酵中的作用。

The purpose of this study is to enhance bacterial hydrogen production. During this research activity, an exhaustive screening using an Escherichia coli mutant library was performed to find a useful gene related to hydrogen production, which could be helpful to fully understand biohydrogen production in E. coli; as a result, there are several uncharacterized genes, which have been never reported that they are related to hydrogen production. In addition, the function of E. coli hydrogenases in hydrogen fermentation from glycerol was studied.