What establishes the symbiotic relationship between human and bifidobacteria?"A bifidogenic effects of human milk oligosaccharides"

是什么建立了人类和双歧杆菌之间的共生关系？“人乳低聚糖的双歧杆菌效应”

• 批准号: 22780072
• 负责人: KATAYAMA Takane
• 金额: $ 2.58万
• 依托单位: Ishikawa Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780072/
• 关键词: ヒト母乳オリゴ糖; ビフィズス菌; 共生; グリコシダーゼ; 母乳オリゴ糖; ビフィズス因子; オリゴ糖代謝; 腸内細菌; ミルクオリゴ糖; ムチン型糖鎖; 精密オリゴ糖合成

The breast-fed infant intestine is often colonized by particular bifidobacteria, and human milk oligosaccharides(HMOs) are considered to be bifidogenic ; however, the precise mechanism underlying it remains unresolved. We have isolated and characterized various glycosidases that act on HMOs from Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. longum and revealed how these enzymes are involved in the degradation of HMOs by these bifidobacteria. These results provide us with essential and basic understanding of physiology of bifidogenic effects of HMOs, and suggested co-evolution of bifidobacteria with humans. We also developed an efficient method of enzymatic synthesis of 2'-fucosyllactose, the most abundant component of HMOs, by introducing a glycosynthase technology into 1, 2-α-L-fucosidase.

母乳喂养的婴儿肠道通常由特定的双歧杆菌定植，人类乳寡糖(HMOS)被认为是双歧的；然而，其背后的确切机制仍未解决。我们已经从双歧双歧杆菌、短双歧杆菌、长双歧杆菌亚种中分离并鉴定了作用于HMOS的各种糖苷酶。婴儿，长双歧杆菌亚种。Long um和揭示了这些酶是如何参与这些双歧杆菌降解HMOS的。这些结果为我们提供了对HMOS双歧效应生理学的基本和基本的了解，并暗示了双歧杆菌与人类的共同进化。我们还通过在1，2-α-L-岩藻糖苷酶中引入糖合成酶技术，开发了一种高效的酶法合成HMOS中含量最丰富的成分2‘-岩藻糖基乳糖的方法。

项目成果

Physiology of the consumption of human milk oligosaccharides by infant-gut associated bifidobacteria

婴儿肠道相关双歧杆菌消耗母乳低聚糖的生理学

• 发表时间: 2011
• 期刊: Journal of Biological Chemistry
• 影响因子: 4.8
• 作者: Asakuma S;Hatakeyama E;Urashima T;Yoshida E;Katayama T;Yamamoto K;Kumagai H;Ashida H;Hirose J;Kitaoka M.
• 通讯作者: Kitaoka M.

ビフィズス菌の共生戦略

双歧杆菌的共生策略

• 发表时间: 2012
• 作者: 片山高嶺;芦田久;熊谷英彦;山本憲二
• 通讯作者: 山本憲二

A UNIQUE PATHWAY OF BIFIDOBACTERIA FOR THE DEGRADATION OF HUMAN MILK OLIGOSACCHARIDES

双歧杆菌降解母乳低聚糖的独特途径

• 发表时间: 2011
• 作者: Takane Katayama;Hisashi Ashida;Motomitsu Kitaoka;Shinya Fushinobu;Hidehiko Kumagai;and Kenji Yamamoto
• 通讯作者: and Kenji Yamamoto

Cooperation of β-galactosidase and β-N-acetylhexosaminidase from bifidobacteria in assimilation of human milk oligosaccharides with type 2 structure

• DOI: 10.1093/glycob/cwq101
• 发表时间: 2010-11-01
• 期刊: GLYCOBIOLOGY
• 影响因子: 4.3
• 作者: Miwa, Mika;Horimoto, Tomohiro;Yamamoto, Kenji
• 通讯作者: Yamamoto, Kenji

Bifidobacterium bifidum由来の1,3-1,4-α-L-フコシダーゼの改変によるルイスa/x抗原の合成

通过修饰两歧双歧杆菌 1,3-1,4-α-L-岩藻糖苷酶合成 Lewis a/x 抗原

• 发表时间: 2011
• 作者: 桜間晴子;吉田永史奈;本多裕司;芦田久;北岡本光;片山高嶺;山本憲二;熊谷英彦
• 通讯作者: 熊谷英彦