The analysis of recognition of pathogen infection and induction of hypersensitive reaction by plant receptor-type protein kinases

植物受体型蛋白激酶识别病原菌感染及诱导过敏反应的分析

• 批准号: 22780088
• 负责人: SAWANO Yoriko
• 金额: $ 2.66万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780088/
• 关键词: 受容体型キナーゼ; 植物病原菌; エリシター; X線結晶構造解析; 核磁気共鳴解析; 相互作用解析; 植物; 受容体キナーゼ; 過敏感反応; 過敏感反応誘導; 病原菌感染認識; 核磁気共鳴

Plants recognize molecules derived from pathogen, elicitor, and cause hypersensitive response(HR) as one of protective reaction against pathogen. Plant cysteine-rich receptor-like kinases have been reported to relate the induction of HR, and its recognition mechanim of pathogen was analyzed in this study. A candidate for elicitor, interacted with CRK, was found in yeast homogenate, and 3-D structure of CRK was solved by X-ray crystallography and the interaction site of CRK was identified by nuclear magnetic resonance spectroscopy.

植物识别病原菌分子、诱导子，引起过敏反应（hypersensitiveresponse，HR），这是植物对病原菌的一种保护性反应。植物富含半胱氨酸受体样激酶与HR的诱导有关，本文分析了其识别病原菌的机制。在酵母匀浆中发现了一个与CRK相互作用的激发子候选物，并通过X射线晶体学解析了CRK的三维结构，通过核磁共振谱确定了CRK的相互作用位点。

项目成果

Nuts and Seeds in Health and Disease Prevention

• DOI: 10.1016/c2009-0-01960-6
• 发表时间: 2011
• 作者: V. Preedy;R. Watson;V. Patel

Cloning of genes and enzymatic characterizations of novel dioscorin isoforms from Dioscorea japonica

薯蓣新亚型薯蓣苷的基因克隆和酶学表征

• 发表时间: 2012
• 期刊: Plant Sci.
• 作者: Xue YL;Miyakawa T;Sawano Y;Tanokura M.
• 通讯作者: Tanokura M.

Crystallization and preliminary X-ray crystallographic analysis of dioscorin from Dioscorea japonica

薯蓣皂甙的结晶及初步X射线晶体分析

• 发表时间: 2012
• 期刊: Acta Crystallogr Sect F Struct Biol Cryst Commun
• 作者: Xue YL;Miyakawa T;Sawano Y;Tanokura M.
• 通讯作者: Tanokura M.