Proposal of new screening method of adhesive probiotic lactic acid bacteria by enzymic activity

我们的目的是建立一个新的筛选方法，高粘附性乳酸杆菌的基础上的酶活性，而不使用粘附试验。测定了30株从人肠组织分离的乳酸杆菌的细胞表面GAPDH活性。GAPDH活性检测在所有样品从18小时的培养物。这表明GAPDH在许多乳酸杆菌的细菌细胞表面普遍表达。由于GAPDH活性与乳酸杆菌的粘附性呈正相关，因此，不通过粘附试验而利用GAPDH酶活性进行筛选是可行的。此外，热休克蛋白GroEL也在许多乳酸杆菌中被检测到。这表明GAPDH和GroEL不仅在细胞质中而且在细胞表面都是非常重要的蛋白，并且可能是乳酸杆菌的通用粘附素。

Our aim was to construct a new screening method for highly adhesive lactobacilli based on an enzymatic activity without using the adhesion test. Thirty lactobacilli isolates from human intestinal tissues were measured for cell surface GAPDH activity. GAPDH activities were detected in all samples from 18 h cultures. This suggests GAPDH is universally expressed on the bacterial cell surfaces from many lactobacilli. Thenew screening method using GAPDH enzymatic activity without an adhesion test may be possible due to the positive correlation of GAPDH activity with adhesion of lactobacilli.Moreover, the heat shock protein, GroEL, was also detected in many lactobacilli. This suggests GAPDH and GroEL are a very important protein not only in the cytoplasm but also on the cell surface and may be a universal adhesin for lactobacilli.