Establishment of multidrug therapy using Atovaquone against canine Babesiosis.

本研究的目的是建立阿托伐醌（atovaquone， ATV）对犬巴贝斯虫病病原菌吉氏巴贝斯虫（Babesia gibsoni）的有效应用。我们用gibsoni连续培养进行了一些实验；(1)线粒体功能分析，(2)分子分析，(3)其他药物敏感性试验，(4)ATV与其他药物对gibsoni的相互作用试验，(5)Malarone(R)对犬急性gibsoni感染的治疗效果评价。结果表明，该原生动物对亚细亚病毒的抗性可能与线粒体细胞色素b基因上的M121I有关，且对亚细亚病毒耐药的gibsoni对其他化学物质没有交叉抗性。ATV与其他药物联合使用对原虫增殖具有协同增效作用。本研究还表明，美拉龙(R)对犬急性gibsoni感染有很高的疗效。

The purpose of this study was to establish an effective usage of atovaquone(ATV) against Babesia gibsoni, which is a pathogen of canine Babesiosis. We performed some experiment using B. gibsoni continuous culture ;(1) analysis of mitochondrial function,(2) molecular analysis,(3) sensitivity test using other chemicals,(4) interaction test among ATV and other drugs against B. gibsoni, and(5) assessment of treatment efficacy of Malarone(R) against canine acute B. gibsoni infection. As the result, it was strongly suggested that the ATV resistance of this protozoa may related to M121I on mitochondorial cytochrome b gene, and also that ATV resistant B. gibsoni have not cross resistance against other chemicals. Combination of ATV and other drugs showed synergic to additive efficacy against the protozoal multiplication. In this study, it was also shown that Malarone(R) have high efficacy against acute B. gibsoni infection in dogs.