Development of specific and highly sensitive method to detect nitroxyl by mass spectrometry

本研究旨在建立一种专属性强、灵敏度高的质谱仪检测硝基(HNO)的方法。合成了一种含有碱性基团以增强MS信号强度的水溶性硫醇化合物。用多反应监测技术(MRM)检测了硫醇化合物与HNO反应生成的亚磺酰胺。我们的结果表明，亚磺酰胺是检测HNO的有用标记物。

This study aimed to develop specific and highly sensitive method to detect nitroxyl(HNO) by mass spectrometry(MS). A water-soluble thiol compound containing basic moiety to enhance MS signal intensity was synthesized. Sulfinamide produced by the reaction of thiol compound with HNO was detected by multiple reaction monitoring(MRM). Our results suggested that sulfinamide is a useful marker for detection of HNO.