Production ofgene-modified animalbytransposase-mediated gene transduction into spermatogonial stem cells.

为了生产基因修饰动物，我们从mdba /2小鼠和大鼠的不同谱系中建立了种系干细胞。以C166内皮细胞为饲养源，建立了C57BL/6小鼠生殖系干细胞。为了评估piggybac介导的基因转导是否适用于种系干细胞，我们通过PiggyBacsystem将EGFP表达载体转导到种系干细胞中。因此，PiggyBac介导的转导可以产生表达强EGFP的稳定的转染物，表明转座酶介导的基因转导对种系干细胞是有效的。

For the production of gene-modified animal, germline stem cells were established fromDBA/2 mice and various lineages of rats. Germline stem cells derived from C57BL/6mice were also established by using C166 endothelial cells as a feeder. To assesswhether PiggyBac-mediated gene transduction could be applicable to germline stemcells, EGFP expression vector was transduced into germline stem cells via PiggyBacsystem. As a result, PiggyBac mediated transduction could produce stabletransfectants that expressed strong EGFP, suggesting that transposase-mediated genetransduction is effective for germline stem cells.