Analysis of the mechanism of virulence factor dependent-inflammasome activation upon Listeria monocytogenes infection

单增李斯特菌感染毒力因子依赖性炎症小体激活机制分析

• 批准号: 22790405
• 负责人: HARA Hideki
• 金额: $ 2.5万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790405/
• 关键词: 感染免疫; Listeria monocytogenes; Listeriolysin O; Inflammasome

Inflammasome activation is responsible for the programmed cell death pytoptosis and cytokine production including IL-1 beta and IL-18. Based on our results, it was clarified that AIM2, type I IFN and kinase signals such as Syk are involved in the inflammasome activation induced by Listeria monocytogenes. Furthermore, we narrowed down the responsible region of listeriolysin O for inflammasome activation as a bacterial factor and revealed that the amino acid region from 204 to 254 of listeriolysin O is important for the activity.

炎性小体活化负责程序性细胞死亡、凋亡和细胞因子产生，包括IL-1 β和IL-18。基于我们的研究结果，它是澄清，AIM 2，I型IFN和激酶信号，如Syk参与炎症小体激活李斯特菌诱导的单核细胞增多症。此外，我们缩小了嗜酸乳杆菌溶血素O作为细菌因子对炎性小体激活的负责区域，并揭示了嗜酸乳杆菌溶血素O的204至254的氨基酸区域对于活性是重要的。

项目成果

Critical roles of ASC inflammasomes in caspase-1 activation and host innate resistance to Streptococcus pneumoniae infection.

• DOI: 10.4049/jimmunol.1100381
• 发表时间: 2011-11-01
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Fang R;Tsuchiya K;Kawamura I;Shen Y;Hara H;Sakai S;Yamamoto T;Fernandes-Alnemri T;Yang R;Hernandez-Cuellar E;Dewamitta SR;Xu Y;Qu H;Alnemri ES;Mitsuyama M
• 通讯作者: Mitsuyama M

Involvement of listeriolysin O-mediated inflammasome activation in bacterial virulence upon Listeria monocytogenes infection

李斯特菌溶血素 O 介导的炎症小体激活参与单核细胞增生李斯特菌感染的细菌毒力

• 发表时间: 2011
• 作者: Hideki Hara;Kohsuke Tsuchiya;Takeshi Yamamoto;Ikuo Kawamura;Masao Mitsuyama
• 通讯作者: Masao Mitsuyama

Involvement of type I interferon in inflammasome formation in macrophages infected with Listeria monocytogenes.

I 型干扰素参与感染单核细胞增生李斯特菌的巨噬细胞中炎症小体的形成。

• 发表时间: 2010
• 作者: H. Hara;K. Tsuchiya;I. Kawamura;T. Yamamoto;T. Nomura and M. Mitsuyama.
• 通讯作者: T. Nomura and M. Mitsuyama.

Involvement of Absent in Melanoma 2 in Inflammasome Activation in Macrophages Infected with Listeria monocytogenes

• DOI: 10.4049/jimmunol.1001058
• 发表时间: 2010-07
• 期刊: The Journal of Immunology
• 作者: K. Tsuchiya;H. Hara;I. Kawamura;T. Nomura;Takeshi Yamamoto;S. Daim;Sita R. Dewamitta;Yanna Shen;Rendong Fang;M. Mitsuyama

Participation of inflammasome activation mediated by Listeriolysin O in bacterial virulence upon Listeria monocytogenes infection.

李斯特菌溶血素 O 介导的炎症小体激活参与单核细胞增生李斯特菌感染的细菌毒力。

• 发表时间: 2011
• 作者: H. Hara;K. Tsuchiya;T. Yamamoto;I. Kawamura and M. Mitsuyama.
• 通讯作者: I. Kawamura and M. Mitsuyama.