Functional analysis of CM2 in influenza C virus replication by using the mutants of the posttranslational modification sites

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To investigate the role(s) of CM2 in the virus replication, we generated and analysed recombinant influenza C viruses lacking the posttranslational modification sites. As a result, the recombinant viruses, in which the glycosylation site or three cysteine residues involved in oligomerization of CM2 were mutated to alanine, grew less efficiently than the wild-type virus. Analyses using virus-like particles revealed that glycosylation of CM2 is involved in the genome packaging and uncoating processes.