Detection of non-culturable Legionella species by amoebal co-culture from bath water samples.

在这项研究中，我们调查了不可培养的军团菌种在洗澡水样品中使用阿米巴共培养。采用阿米巴菌共培养法对71份水样进行军团菌检测。采用阿米巴菌与培养法、PCR法和定性PCR法共培养洗澡水63份（88.7%）阳性，常规培养法12份（16.9%）阳性。结果表明，洗澡水样品被军团菌（包括VBNC型）高度污染。

In this study, we investigated the prevalence of non-culturable Legionella species in bath water samples using amoebal co-culture. Legionella species were detected by amoebal co-culture method from 71 water samples. A total of 63(88.7%) bath water samples were positive by amoebal co-culture with culture, PCR, and qualitative PCR methods, whereas 12(16.9%) water samples were positive by conventional culture method. Our results suggested that bath water samples were highly contaminated by Legionella bacteria including VBNC form.