Detection of non-culturable Legionella species by amoebal co-culture from bath water samples.

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In this study, we investigated the prevalence of non-culturable Legionella species in bath water samples using amoebal co-culture. Legionella species were detected by amoebal co-culture method from 71 water samples. A total of 63(88.7%) bath water samples were positive by amoebal co-culture with culture, PCR, and qualitative PCR methods, whereas 12(16.9%) water samples were positive by conventional culture method. Our results suggested that bath water samples were highly contaminated by Legionella bacteria including VBNC form.