Role of endothelial lipase in anti-inflammatory properties of high-density lipoprotein

内皮脂肪酶在高密度脂蛋白抗炎特性中的作用

• 批准号: 22790705
• 负责人: HARA Tetsuya
• 金额: $ 2.5万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790705/
• 关键词: 炎症; 動脈硬化; 脂質代謝; 血管内皮; リパーゼ; 高比重リポ蛋白質; 循環器; 分子血管病態学; 生活習慣病; 高比重リポ蛋白

Inhibition of endothelial lipase(EL) increases plasma high-density lipoprotein cholesterol(HDL-C) level. In this study, we assessed the functional quality of HDL isolated from EL-/-and WT mice. Anti-inflammatory functions of HDL, such as PON-1 or PAF-AH activities, inhibition of cytokine-induced VCAM-1 expression, inhibition of LDL oxidation, were similar between WT and EL-/-mice. In contrast, lipopolysaccharideneutralizing capacity of HDL was higher in EL-/-mice than in WT mice. Intravenous injection of HDL isolated from EL-/-mice improved the mortality in LPS-treated mice. Thus, EL inactivation increased plasma HDL particles with preserved anti-inflammatory and anti-atherosclerotic functions.

抑制内皮脂肪酶（EL）可增加血浆高密度脂蛋白胆固醇（HDL-C）水平。在这项研究中，我们评估了从EL-/和WT小鼠中分离的HDL的功能质量。HDL的抗炎功能，如PON-1或PAF-AH活性，抑制细胞因子诱导的VCAM-1表达，抑制LDL氧化，在WT和EL-/-小鼠之间相似。相反，EL-/-小鼠的HDL脂多糖中和能力高于WT小鼠。静脉注射EL-/-小鼠体内分离的HDL可改善lps处理小鼠的死亡率。因此，EL失活增加了血浆HDL颗粒，保留了抗炎和抗动脉粥样硬化功能。

项目成果

Establishment of a novel, highsensitive ELISA system for human endothelial lipase

新型高灵敏人内皮脂肪酶ELISA系统的建立

• 发表时间: 2012
• 作者: Ishida;T;Miyashita;K;Kinoshita;N;Seito;S;Hirata K;Nakajima K.
• 通讯作者: Nakajima K.

Cardiac endothelial lipase expression is augmented in the early stage of heart failure

心力衰竭早期心脏内皮脂肪酶表达增强

• 发表时间: 2012
• 作者: Hideto Nakajima;Tatsuro Ishida;Maki Sasaki;Kensuke Kondo;Kenta Mori;Minoru Hasokawa;Yoko Haraguchi;Tomoyuki Yasuda;Seimi Kobayashi;Ken-ichi Hirata.
• 通讯作者: Ken-ichi Hirata.

Endothelial cell-selective adhesion molecule modulates atherosclerosis through plaque angiogenesis and monocyte-endothelial interaction

• DOI: 10.1016/j.mvr.2010.04.005
• 发表时间: 2010-09-01
• 期刊: MICROVASCULAR RESEARCH
• 影响因子: 3.1
• 作者: Inoue, Michihiko;Ishida, Tatsuro;Hirata, Ken-ichi
• 通讯作者: Hirata, Ken-ichi

Cardiac endothelial lipase expression is augmented in a murine model of heart failure

心力衰竭小鼠模型中心脏内皮脂肪酶表达增强

• 发表时间: 2012
• 作者: Nakajima H;et al
• 通讯作者: et al

Rader DJ. Update on the role of endothelial lipase in HDL metabolism, reverse cholesterol transport, and atherosclerosis

雷德 DJ。

• 发表时间: 2010
• 期刊: Circ J
• 影响因子: 3.3
• 作者: Yasuda T;Ishida T
• 通讯作者: Ishida T