The search for sterss-induced conditioning: effects of acute hypercapnia

寻找应激诱导的调节：急性高碳酸血症的影响

• 批准号: 23592257
• 负责人: HARA Tetsuya
• 金额: $ 3.24万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592257/
• 关键词: 心筋虚血; コンディショニング; 高炭酸ガス血症; 気絶心筋; 高二酸化炭素血症; 心筋虚血再灌流傷害; 心筋スタニング; 虚血耐性; プレコンディショニング; ポストコンディショニング; 周術期管理

We studied the cardioprotective effect of acute hypercapnia before ischemia or during reperfusion (pre- or post-conditioning) in swine stunned myocardium. Hypercapnia was induced by hypoventilation of PaCO2 70 mmHg for 30 minutes. Stunning was produced by 12-min ischemia and 90-min reperfusion. Myocardial contractility was evaluated with segment shortening. Data were analyzed with Mann-Whitney U test. p<0.05 was considered statistically significant. PaCO2 and coronary blood flow during hypercapnia were significantly higher than those in control swine. There was no significant recovery of stunning. Interleukin after reperfusion were slightly attenuated in pre-conditioned swine. KATP channels were not activated during hypercapnia. Acute hypercapnia could not enhance recovery of stunned myocardium. Attenuation of the preconditioning stimuli by the increase of coronary blood flow would be considered as the result, and therefore enough conditioning stimuli would not be obtained.

我们研究了猪顿抑心肌缺血前或再灌注期间（预处理或后处理）急性高碳酸血症的心脏保护作用。通过PaCO 2 70 mmHg通气不足30分钟诱导高碳酸血症。12分钟缺血和90分钟再灌注产生眩晕。心肌节段缩短法评价心肌收缩力。数据用Mann-Whitney U检验进行分析。p<0.05被认为具有统计学显著性。高碳酸血症时PaCO 2和冠脉血流量显著高于对照组。昏迷无明显恢复。预处理猪再灌注后白细胞介素略有减弱。KATP通道在高碳酸血症时不被激活。急性高碳酸血症不能促进顿抑心肌的恢复。结果是冠状动脉血流量增加导致预适应刺激减弱，因此无法获得足够的预适应刺激。

项目成果

Effects of acute hypercapnia in stunned myocardium in swine

急性高碳酸血症对猪心肌顿抑的影响

• 发表时间: 2014
• 作者: Tetsuya Hara;Daiji Akiyama;Osamu Yoshitomi
• 通讯作者: Osamu Yoshitomi

高二酸化炭素血症が気絶心筋の回復に与える影響

高碳酸血症对顿挫心肌恢复的影响

• 发表时间: 2014
• 作者: 山本裕梨;荒木寛;諸岡秋葉;穐山大治;吉富修;原哲也
• 通讯作者: 原哲也

再灌流時の高二酸化炭素血症がブタ気絶心筋の回復に与える影響

再灌注期间高碳酸血症对顿顿猪心肌恢复的影响

• 发表时间: 2014
• 作者: 原哲也;一ノ宮大雅;松本周平;趙成三
• 通讯作者: 趙成三