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非被覆性コケムシの群体形態多様化の解明および環境評価への応用に向けた研究

旨在阐明无涂层苔藓虫菌落形态多样性并将其应用于环境评估的研究

基本信息

批准号：
11J03410
负责人：
広瀬雅人
金额：
$ 1.02万
依托单位：
National Museum of Nature and Science, Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

项目摘要

1.スナツブコケムシ科の系統分類本年度は,得られた塩基配列情報に基づいてスナツブコケムシ科の唇口目内における系統的位置を探るとともに,砂泥底に生息するLanceoporidae科Lanceopora属の2種について遺伝的距離を求め,個虫形態が酷似したこれら2種の分類に関する検討を行なった.スナツブコケムシ科2属4種の18SrDNAおよび16SrDNAの塩基配列を決定し,他の唇口目コケムシ類の塩基配列と併せて系統解析を行なった結果,スナツブコケムシ科は単系統群となったが,Lepraliomorpha(下目)については先行研究と同様に単系統群とはならなかった.Lanceopora属2種については18SrDNA,16SrDNA,COIの塩基配列を決定し,前述の系統解析に加えた.その結果,これら2種間に明瞭な遺伝的差異がみられた.そのため,この2種については群体の外見と生活様式の違いが分類形質となり得ることが示された,本年度はこのほかに南西諸島海域およびこれまで調査されてこなかった山陰沖(隠岐)での追加標本の採集も行ない,山陰沖の日本海における本科の棲息をはじめて確認した.2.安定同位体を用いた大型起立性コケムシ群体の成長率の推定本年度は,昨年の分析で速度論的効果がみられた南西諸島海域のニセツノコケムシ群体を対象に,試料採取方法を改変して新たに同位体比分析を行なった,分析に用いる枝について,枝の中心部と表面に分けてサンプリングを行なった結果,枝の中心部における炭素同位体比はほぼ同位体平衡下で形成された値を示したのに対し,枝の表面ではその値が大きく外れ,生物学的要因による同位体効果がはたらいている可能性が示唆された.このことから,ニセツノコケムシ群体においては,枝の中心部は同位体平衡下で形成されている可能性が高く,今後の分析にも有用であると考えられる.また,枝の表面では中心部に比べて酸素同位体比がわずかに軽い傾向もみられたことから,ニセツノコケムシ群体の枝の表面では中心部に比べて炭酸カルシウム骨格の成長速度が速い可能性も考えられる.

1. In this year's project, we have obtained information on the location of the system in the mouth and mouth of the family, the location of the system in the mouth and mouth of the family, the Lanceoporidae family in sand and mud, the Lanceopora of the family, and the distance between them, and the shape of the worm is very similar to that of the two categories. In this family, 2 genera and 4 years of the 18SrDNA family, the basic configuration of the 16SrDNA system has been determined, he has analyzed the results of the system analysis of the system, and the population of the system of the family has been studied first. The system of two species of the genus Lanceopora has been studied with 18s rDNA and 16s rDNA The COI system is based on the column decision, and the aforementioned system is analyzed and added. The results show that the difference between two weeks is clear. In the current year, there is an increase in the number of students in the south-west coast of the sea. This year, please add to this collection of documents, please make sure that you confirm it. 2. The growth rate of this year's population is inferred on the basis of the growth rate of the large-scale standing population. Last year, we analyzed the results of the speed theory in the south and west of the sea, the population in the south and west of the sea, the population in the sea area, the population, the growth rate, the growth rate The central part of the branch is divided on the surface. The results show that the carbon isotope ratio in the central part of the branch shows that under the balance of carbon isotope ratio, it is formed in the balance of carbon isotope. The surface of the branch shows that it is not easy to grow, and the biological reason is that it is possible to show that it is possible to do so. In the middle of the branch, under the balance of the appositive body, the possibility is high. In the future, the analysis will be useful. In the center of the branch surface, the ratio of acid alleles is higher than that of acid alleles, and the growth rate of the central part of the surface is faster than that of carbon acid.