Development of intestinal regeneration in vivo using polyglycolic acid felt as a extracellular matrix.

使用聚乙醇酸作为细胞外基质开发体内肠道再生。

• 批准号: 22591983
• 负责人: KIMURA Osamu
• 金额: $ 2.83万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591983/
• 关键词: 小腸機能不全; 小腸再生; 神経ペプチド; ポリグリコール酸フェルト; 胎児肺低形成; 肺再生治療

The aim of this study was intestinal regeneration using neuropeptide bombesin in rats. But it was elucidated that administration of bombesin was not enough to regenerate muscle layer properly with normal mucosal structure. Because of the limit of time and cost, we changed the purpose of this project from intestinal regeneration to lung regeneration with the same neuropeptide using CDH rat model.Using pregnant Sprague-Dawley (SD) rat(term22) which received 100mg of nitrofen into stomach through nasogastric tube on day 9.5, it was found that lung weight to body weight ratio(LW/BW) in CDH rat increases bombesin dose-dependently.

本研究的目的是利用神经肽蛙皮素对大鼠进行肠道再生。但已有研究表明，蛙皮素的应用不足以使肌层正常再生，并具有正常的粘膜结构。由于时间和成本的限制，我们使用相同神经肽的CDH大鼠模型，将该项目的目的从肠道再生改为肺再生。使用怀孕的Spraogue-Dawley(SD)大鼠(Term22)，在第9.5天通过鼻胃管给予100 mg硝芬，发现CDH大鼠的肺重量/体重比(LW/BW)增加，并呈剂量依赖关系。

项目成果

Impact of our new protocol on the outcome of the neonates with congenital diaphragmatic hernia

• DOI: 10.1007/s00383-012-3242-z
• 发表时间: 2013-04-01
• 期刊: PEDIATRIC SURGERY INTERNATIONAL
• 影响因子: 1.8
• 作者: Kimura, Osamu;Furukawa, Taizo;Tajiri, Tatsuro
• 通讯作者: Tajiri, Tatsuro