Nuclear DNA content in carcinoma of the stomach, colon and rectum with biologically high malignancy

生物学高度恶性的胃癌、结肠癌和直肠癌的核 DNA 含量

• 批准号: 63570635
• 负责人: KIMURA Osamu
• 金额: $ 1.02万
• 依托单位: Tottori University School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570635/
• 关键词: Nuclear DNA Content; Gastric Cancer; Colorectal Cancer; Biological Malignancy; Preoperative Diagnosis; Ploidy Pattern; Flowcytometer; Microspectrophotometer

Nuclear DNA content was microspectrophotometrically measured in 151 gastric carcinomas and 23 colorectal carcinomas, to assess the predominance of a particular DNA distribution pattern in carcinoma of the stomach, colon and rectum with biologically high malignancy, such as the marked tendency for severe vessel invasion and remote metastasis, and for early recurrence after curative surgery. On the other hand, nuclear DNA content was flowcytometrically analyzed in 12 gastric carcinomas and 6 colorectal carcinomas both in biopsy specimen from endoscopy and in resected- specimen, to investigate correspondence between them.The incidence of high DNA ploidy was higher in cases with severe vessel invasion and remote metastasis among cases of the same depth of invasion, and in dead cases of early recurrence among cases of the same histological stage. Furthermore, there was a close correspondence in DNA ploidy pattern between biopsy and resected specimen except one case.Therefore, we consider that the DNA distribution pattern well reflects the biological malignancy in carcinoma of the stomach, colon and rectum. Further, examination of the DNA ploidy in preoperative biopsy may offer a good clue to the appropriate choice of treatment of carcinoma according as its biological malignancy.

用显微分光光度法检测151例胃癌和23例结直肠癌的细胞核DNA含量，以探讨一种特殊的DNA分布模式在恶性程度较高的胃癌、结肠癌和直肠癌中的优势，如严重血管侵袭和远处转移的明显倾向以及根治手术后早期复发的可能性。另一方面，对12例胃癌和6例结直肠癌的胃镜活检标本和手术切除标本进行了DNA含量的流式细胞术分析，以探讨两者之间的相关性。在同一组织学分期中，血管侵犯严重、远处转移的病例DNA倍体率较高，早期复发死亡病例DNA倍体较高。此外，除1例外，活检标本与手术切除标本的DNA倍体图谱有较好的一致性，因此，我们认为DNA分布图谱较好地反映了胃癌、结肠癌和直肠癌的生物学恶性程度。此外，术前活检中DNA倍体的检测可能为根据肿瘤的生物学恶性程度选择合适的治疗方法提供很好的线索。

项目成果

広岡保明: 米子医学雑誌. 39(2). 80-91 (1988)

广冈康明：米子医学杂志 39(2)（1988）。

木村修: "DNA ploidy in gastric cancer with metastasis to the liver" Journal of Surgical Oncology.

Osamu Kimura：“胃癌肝转移中的 DNA 倍体”《肿瘤外科杂志》。

村田裕彦: "細胞核DNA量からみた腫瘍原発巣と転移巣の生物学的特性に関する比較的検討" 米子医学雑誌. 39(2). 92-102 (1988)

Hirohiko Murata：“基于核 DNA 量的原发性肿瘤和转移性肿瘤的生物学特征的比较研究”米子医学杂志 39（2）（1988）。

Murata Yuhiko: "A cytophotometric study on the nuclear DNA content of primary and metastatic lesions of experimental animal tumors." J. Yonago Med. Ass., 39(2), 92-102, 1988 (in Japanese).

Murata Yuhiko：“实验动物肿瘤原发性和转移性病变的核 DNA 含量的细胞光度研究。”

木村修: "早期胃癌再発症例における腫瘍細胞核DNAの量の検討" 日本消化器外科学会雑誌.

木村修：“早期胃癌复发病例中肿瘤细胞核DNA量的检查”日本胃肠外科学会杂志。