A fundamental study of development of the wound healing therapy focusing on the characteristic of myofibroblast.

以肌成纤维细胞特性为重点的伤口愈合治疗发展的基础研究。

• 批准号: 22591989
• 负责人: ABE Masatoshi
• 金额: $ 1.25万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591989/
• 关键词: 創傷治癒; 細胞生物学; 筋線維芽細胞; 増殖因子; アポトーシス; Akt; 線維芽細胞; b-FGF; MCP-1; 細胞内シグナル伝達; ケモカイン; ケロイド

Although recent clinical reports have indicated that recombinant basic fibroblast growth factor (bFGF) promotes scarless wound healing, the mechanism remains unclear. The present study was carried out to elucidate the mechanisms. The protein levels of cellular α-smooth muscle actin increased at 2-4 days after TGFβ treatment alone and at 4 to 6 days after a costimulation of bFGF and TGFβ. A spontaneous contraction of stressed myofibroblast-collagen matrix was cancelled by bFGF, which was restored under the presence of C3 exotransferase or Y27632. bFGF stimulation of myofibroblasts as well as fibroblasts elicited a transient Rac and Rho activation. bFGF promoted apoptosis of the myofibroblasts but not of the fibroblasts, even in the presence of two different inhibitors, either LY294002 or an Akt inhibitor. The present study suggests that the phosphatidylinositol-3-kinase to Akt as well as the Rho to Rho kinase signaling pathway is involved in bFGF-promoted myofibroblast apoptosis, and bFGF can promote the scarless wound healing upon the induction of apoptosis of myofibroblasts, but not fibroblasts.

尽管最近的临床报道表明，重组碱性成纤维细胞生长因子(BFGF)促进无瘢痕创面愈合，但其机制尚不清楚。本研究旨在阐明其作用机制。细胞α-平滑肌肌动蛋白的蛋白水平在转化生长因子β单独作用后2-4天和联合刺激后4-6天升高。应激肌成纤维细胞-胶原基质的自发收缩可被碱性成纤维细胞生长因子取消，在C3外转移酶或Y27632存在下恢复。碱性成纤维细胞生长因子对肌成纤维细胞和成纤维细胞的刺激可引起短暂的Rac和Rho激活。即使在两种不同的抑制剂LY294002或Akt抑制剂存在的情况下，碱性成纤维细胞生长因子也能促进肌成纤维细胞的凋亡，但不能促进成纤维细胞的凋亡。本研究提示，bFGF诱导的肌成纤维细胞的凋亡与磷脂酰肌醇-3-肌醇-Akt和Rho-Rho的信号通路有关，bFGF在诱导成纤维细胞凋亡时可促进无瘢痕创面的愈合，但对成纤维细胞无此作用。

项目成果

やさしいキズのみかたと局所療法の選択

治疗划痕的简单方法并选择局部疗法

• 发表时间: 2011
• 作者: Abe M;et al.;安部正敏;安部正敏
• 通讯作者: 安部正敏

Effects of Cyclosporin A on extracellular matrix metabolism by human dermal fibroblasts.

环孢素 A 对人真皮成纤维细胞细胞外基质代谢的影响。

• 发表时间: 2012
• 作者: Abe M;Yokoyama Y;Syuto T;Ishikawa O.
• 通讯作者: Ishikawa O.

皮膚の見方 ナビカード

如何查看皮肤导航卡

• 发表时间: 2011
• 作者: Abe M.;et al.;安部正敏;安部正敏
• 通讯作者: 安部正敏

骨・筋肉・皮膚イラストレイティッド

骨骼、肌肉和皮肤插图

• 发表时间: 2010
• 作者: 安部正敏;窪田誠編
• 通讯作者: 窪田誠編

Successful treatment of combination therapy with tacalcitol lotion associated with sunscreen for localized Darier's disease

• DOI: 10.1111/j.1346-8138.2010.00910.x
• 发表时间: 2010-08-01
• 期刊: JOURNAL OF DERMATOLOGY
• 影响因子: 3.1
• 作者: Abe, Masatoshi;Yasuda, Masato;Ishikawa, Osamu
• 通讯作者: Ishikawa, Osamu