Establishment of cytological diagnosis and HPV detection system of oral squamous cell carcinoma and precancerous lesion

约100例疑似鳞状细胞癌或癌前病变的细胞学诊断几乎全部正确。阳性病例均为鳞状细胞癌，阴性病例均为良性病变。其中50例未检出HPV-DNA。采用免疫组织化学双染色法检测细胞学涂片中p53蛋白和p16蛋白。p53阳性检测癌细胞的敏感性为30%，特异性为100%。p16蛋白的敏感性和特异性均不显著。

The cytological diagnoses for about 100 cases of suspected squamous cell carcinomas or precancerous lesions were almost all correct. All of positive cases were squamous cell carcinomas, and negative cases were benign lesions. HPV-DNA was not detected in 50 cases of these lesions. Immunohistochemical double staining method was used for detecting p53 protein and p16 protein in cytological smears. The sensitivity of p53 positivity for detection of cancer cells was 30%, and its specificity was 100%. The sensitivity and specificity of p16 protein were not significant.