Establishment of cytological diagnosis and HPV detection system of oral squamous cell carcinoma and precancerous lesion

口腔鳞癌及癌前病变细胞学诊断及HPV检测体系的建立

• 批准号: 22592034
• 负责人: KISHINO Mitsunobu
• 金额: $ 2.33万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592034/
• 关键词: 扁平上皮癌; 前癌病変; HPV; 細胞診; 免疫染色; ＨＰＶ

The cytological diagnoses for about 100 cases of suspected squamous cell carcinomas or precancerous lesions were almost all correct. All of positive cases were squamous cell carcinomas, and negative cases were benign lesions. HPV-DNA was not detected in 50 cases of these lesions. Immunohistochemical double staining method was used for detecting p53 protein and p16 protein in cytological smears. The sensitivity of p53 positivity for detection of cancer cells was 30%, and its specificity was 100%. The sensitivity and specificity of p16 protein were not significant.

约100例疑似鳞状细胞癌或癌前病变的细胞学诊断几乎全部正确。阳性病例均为鳞状细胞癌，阴性病例均为良性病变。其中50例未检出HPV-DNA。采用免疫组织化学双染色法检测细胞学涂片中p53蛋白和p16蛋白。p53阳性检测癌细胞的敏感性为30%，特异性为100%。p16蛋白的敏感性和特异性均不显著。

项目成果

フォーラム「口腔扁平上皮癌の細胞診」口腔扁平上皮癌および前癌病変における HPV の関与について

《口腔鳞状细胞癌细胞学》论坛关于HPV与口腔鳞状细胞癌及癌前病变的关系

• 发表时间: 2012
• 作者: Ishibashi M;Kishino M;Sato S;Morii E;Ogawa Y;Aozasa K;Kogo M;Toyosawa S;Miki Ishibashi;岸野万伸
• 通讯作者: 岸野万伸

The prevalence of human papillomavirus in oral premalignant lesions and squamous cell carcinoma in comparison to cervical lesions used as a positive control

与用作阳性对照的宫颈病变相比，口腔癌前病变和鳞状细胞癌中人乳头瘤病毒的患病率

• 发表时间: 2011
• 期刊: Int J Clin Oncol
• 影响因子: 3.3
• 作者: Ishibashi M;Kishino M;Morii E;Ogawa Y;Aozasa K;Kogo M;Toyosawa S.
• 通讯作者: Toyosawa S.

フォーラム「口腔扁平上皮癌の細胞診」口腔扁平上皮癌および前癌病変におけるHPVの関与について

《口腔鳞状细胞癌细胞学》论坛关于HPV与口腔鳞状细胞癌及癌前病变的关系

• 作者: Ishibashi M;Kishino M;Sato S;Morii E;Ogawa Y;Aozasa K;Kogo M;Toyosawa S;Miki Ishibashi;岸野万伸;岸野万伸
• 通讯作者: 岸野万伸