A study of concavo-convex formation mechanism in occlusal surface by using of TDL culture.

本研究最初计划采用TDL（三维分层）培养法和浸泡珠法相结合的方法来研究咬合面凹凸的形成机制。然而，由于原代细胞的使用，使实验变得复杂，而且我们不容易获得足够的样品。因此，我们尝试改进方法如下：1.细胞系的使用，2. TDL方法的周转，3。开发新的分析方法。结果，我们发现了一个具有有趣特征的细胞系，并发现了几种新的方法。

Initially, we had a plan in which we use the combination of TDL (Three dimensional & layered) culture method and soaked-beads method for the study of concavo-convex formation mechanism in occlusal surface. However, the protocol became complexity for the usage of primary cells, and we could not easy to obtain enough sample. So, we tried to improve methods as follows, 1. Usage of cell-lines, 2. Turnaround of TDL method, 3. Development of new method of analysis. As a result, we found a cell-lineshowing interesting character, and several new methods.