Molecular analysis of craio-facial abnormalities found in the Sez12 knockout mice

在这项研究中，我们研究了纯合Sez 12敲除小鼠（Sez 12-KO小鼠）中显示的表型与敲入eGFP表达分布之间的关系。我们证明了Sez 12在骨骼生长中起着重要的作用，如胚胎发育过程中的颅神经嵴发育和出生后的软骨内骨化。此外，Sez 12上调影响了在血清耗竭等条件下的细胞存活。这些发现表明，Sez 12负责细胞分化和维持分化细胞，导致小鼠骨骼异常，包括颅面畸形。我们分析了口腔手术后患者的神经病理性疼痛和心理结局。

In this study, we investigated the relationship between the phenotype shown in the homozygous Sez12 knockout mice (Sez12-KO mice) and distribution of the knockin eGFP expression. We demonstrate that the Sez12 plays an important role in skeletal growth, such as cranial neural crest development during embryogenesis and endochondral ossification after birth. Furthermore, the Sez12 upregulation affected cell survival under a condition such as serum depletion. These findings suggest that the Sez12 is responsible for cell differentiation and maintenanceof the differentiating cells, which cause skeletal abnormalities in mice including craniofacial malformation.We analyzed the neuropathic pain and psychological outcome of patients following oral surgery.