stablishment of cell therapy in maxillary and mandibular bone tissue regeneration using canine induced pluripotent stem cells

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We reported the generation of canine induced pluripotent stem cells by the transcription factors Oct3/4, Sox2, Klf4, and c-Myc into canine embryonic fibroblasts (CEFs). Canine iPS cells were cultured on MEFs under knockout serum replacement and neural stem cell serum free medium. Then, flow cytometry was performed on neural-like cells derived from canine iPS cells to sort for p75 and HNK1. Isolated neural crest (NC) cells were cultured in neural stem cell serum free medium containing osteoblast differentiation factor. NC cells isolated from neural-like cell were observed for differentiation based on p75 and HNK1 staining. ALP activity of NC cells was increased in the osteoblast-induction for 14 days, thus, NC cells might be differentiated to osteoblast-like cells.