Assessment of in vitro estrogenic activity of dental materials and its metabolites

牙科材料及其代谢物的体外雌激素活性评估

• 批准号: 12671914
• 负责人: HASHIMOTO Yoshiya
• 金额: $ 2.11万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671914/
• 关键词: estrogenic activity; dental materials; metabolism; in vitro; xenoestrogenic; bisphenol-A; phthalate esters; 義歯粘膜調整材; 義歯軟性裏装材

The estrogenic activities of 51 dental materials related to chemicals, including bisphenol-A (BPA) related chemicals, were examined by three in vitro assays in the presence and absence of a post-mitochondrial metabolizing system (S9 mix). Bisphenol-A -dimethacrylate (Bis-DMA), plasticizers and commercial tissue conditioners showed estrogenic activities. The structure-activity relationships of bisphenol-A related chemicals were found in such a structures involving phenol and the activity was enhanced with S9 mix. Metabolites of BPA, Bis-DMA and Bis-GMA with S9 mix were detected by HPLC, and Bis-DMA was considered to be converted into bisphenol-A. 2- (3, 4-dihydroxyphenol) -2- (4-hydroxyphenol), and therefore yielded estrogenic activitiesTwo aromatic dimethacrylates synthesized from bis (4-hydroxyphenyl) sulfone (BPS) and bis (4-hydroxyphenyl) ketone (BPO) showed little estrogenicity. They also revaealed comparable cell responses to the dental pulp cells with these materials synthesized from BPA (Bis-GMA). A prototype polymer made of the biocompatible aromatic dimethacrylates synthesized from BPS was tested for knoop hardness and found comparable to that of Bis-GMA. This finding gives useful information about the research and development of new dental materials, which are estrogenic activity free and of higher clinical performance. Additional studies, including in vivo and various properties, are required

在存在和不存在线粒体后代谢系统（S9 mix）的情况下，通过三项体外测定检查了 51 种与化学物质（包括双酚 A (BPA) 相关化学物质）相关的牙科材料的雌激素活性。双酚A-二甲基丙烯酸酯（Bis-DMA）、增塑剂和商业组织调理剂显示出雌激素活性。在涉及苯酚的结构中发现了双酚A相关化学物质的构效关系，并且S9 mix增强了活性。通过HPLC检测到BPA、Bis-DMA和Bis-GMA与S9混合物的代谢物，并且Bis-DMA被认为转化为双酚-A。 2-（3, 4-二羟基苯酚）-2-（4-羟基苯酚），因此产生雌激素活性。由双（4-羟基苯基）砜（BPS）和双（4-羟基苯基）酮（BPO）合成的两种芳香族二甲基丙烯酸酯几乎没有表现出雌激素活性。他们还用 BPA（Bis-GMA）合成的这些材料重新研究了牙髓细胞的类似细胞反应。对由 BPS 合成的生物相容性芳香族二甲基丙烯酸酯制成的原型聚合物进行了努普硬度测试，结果发现其与 Bis-GMA 相当。这一发现为新型牙科材料的研究和开发提供了有用的信息，这些材料不含雌激素活性且具有更高的临床性能。需要进行更多研究，包括体内和各种特性

项目成果

橋本典也: "Estrogenic activity of chemicals for dental and similar use it vitro"J Mater Sci Mater in Med. 11. 465-468 (2000)

Noriya Hashimoto：“牙科化学品的雌激素活性和体外类似用途”J Mater Sci Mater in Med. 11. 465-468 (2000)

Yoshiya HASHIMOTO: "Estrogenic activity of tissue confitioners in vitro"Dental Materials. (in press).

Yoshiya HASHIMOTO：“组织调节剂的体外雌激素活性”牙科材料。

橋本典也: "Measurement of estrogenic activity of chemicals for the development of new dental polymers"Toxicology in Vitro. 15. 421-425 (2001)

Noriya Hashimoto：“用于开发新型牙科聚合物的化学品雌激素活性的测量”体外毒理学 15. 421-425 (2001)。

橋本典也: "Estrogenic activity of dental materials and bisphenol-A related chemicals (in vitro)"Dent Mater J. 19. 245-262 (2000)

Noriya Hashimoto：“牙科材料和双酚 A 相关化学物质的雌激素活性（体外）”Dent Mater J. 19. 245-262 (2000)

橋本典也, 川口稔, 宮崎光治, 中村正明: "Measurement of estrogenic activity of chemicals for the development of new dental polymers"Toxicology in Vitro. 15. 421-425 (2001)

Noriya Hashimoto、Minoru Kawaguchi、Mitsuharu Miyazaki、Masaaki Nakamura：“用于开发新型牙科聚合物的化学品雌激素活性的测量”体外毒理学 15. 421-425 (2001)。