Development of dental biosensor using nano-technology

利用纳米技术开发牙科生物传感器

• 批准号: 17592054
• 负责人: HASHIMOTO Yoshiya
• 金额: $ 2.24万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592054/
• 关键词: quartz crystal microbalance; ArF excimer laser; apatite; biosensor; nano-technology; 水晶振動子マイクロバランス; ハイドロキシパタイト

This study examined in vitro cytocompatibility and in situ measurements of protein adsorption using the quartz crystal microbalance (QCM) to thin hydroxyl apatite (HA) and natural apatite (NA) films deposited by laser ablation, in order to investigate better surface conformation of titanium dental implants.Thin HA and NA films on titanium discs and the QCM sensor were made using an ArF excimer laser operating at a repletion rate of 10 Hz and annealed by heat radiation at 360℃ for 1 h. After annealing, the chemical composition of the HA film was characterized by X-ray diffraction (XRD). The surface conformation of the HA and NA films on the titanium disc was observed by energy dispersive analysis (EDX). Subsequently, primary mouse osteoblast (PMO) was cultured on the discs with or without film. Cell proliferation and differentiation were investigated by DNA assay, alkaline phosphatase activity (ALP) assay and osteocalcin production (OCN) assay. The affinity between the films and several proteins in the phosphate buffer was monitored using a 27-Hz QCM.XRD showed crystalline HA and NA films on the Ti and QCM sensor under the annealing conditions. EDX showed peaks of Na and Mg in addition to Ca and P for the NA film. PMO grew faster and had higher ALP and OCN when grown in NA films than on HA films or Ti. The absorbed amount of several proteins on the QCM sensor with NA film was significantly higher than that of HA film and non-film.Based on these findings, NA film deposited by laser ablation shows excellent cytocompatibility with cells owing to the superior absorption of protein. The results obtained could lead to the development of more effective dental implants.

本研究采用石英晶体微天平（QCM）对激光烧蚀沉积的羟基磷灰石（HA）和天然磷灰石（NA）薄膜进行了体外细胞相容性研究和蛋白质吸附的原位测量，为了研究钛种植体的良好表面形态，采用ArF准分子激光器在钛种植体表面制备HA和NA薄膜，并制作QCM传感器在360℃下热辐射1h。退火后，通过X射线衍射（XRD）对HA薄膜的化学成分进行了表征。用能谱分析（EDX）观察了HA和NA膜在钛盘上的表面形态。随后，原代小鼠成骨细胞（PMO）培养的光盘或无膜。通过DNA含量测定、碱性磷酸酶活性测定和骨钙素生成测定观察细胞增殖和分化情况。使用27-Hz QCM监测膜与磷酸盐缓冲液中的几种蛋白质之间的亲和力。XRD显示在退火条件下Ti和QCM传感器上的结晶HA和NA膜。对于NA膜，除了Ca和P之外，EDX还显示Na和Mg的峰。PMO生长速度快，有较高的ALP和OCN时，生长在NA膜比HA膜或Ti。带有NA膜的QCM传感器上多种蛋白质的吸收量明显高于HA膜和非膜，基于这些发现，激光烧蚀沉积的NA膜由于对蛋白质的上级吸收而显示出与细胞良好的细胞相容性。所获得的结果可能会导致更有效的牙科植入物的发展。

项目成果

Biocompatibility of implant with natural apatite ultra-thin film

天然磷灰石超薄膜植入物的生物相容性

• 发表时间: 2006
• 期刊: Journal of Japanese Society for Biomaterials 24
• 作者: HONTSU S;KAWASHIMA M;NISHIKAWA H;KUSUNOKI M;HAYAMIT;OHASHI Y;HIGUCHI Y;HASHIMOTO Y
• 通讯作者: HASHIMOTO Y

生体アパタイト超薄膜被覆インプラントの生体親和性

生物磷灰石超薄膜涂层植入体的生物相容性

• 发表时间: 2006
• 期刊: バイオマテリアル 24・5
• 作者: 本津茂樹;樋口裕一;他6名
• 通讯作者: 他6名

Improvement of hydroxyapatite deposition on titanium dental implant using ArF laser ablation -It's effect on osteoblast biocompatibility in vitro-

ArF激光烧蚀改善钛牙种植体上羟基磷灰石沉积-对体外成骨细胞生物相容性的影响-

• 发表时间: 2006
• 期刊: ADVANCES IN SCIENCE AND TECHNOLOGY 49
• 作者: HASHIMOTO Y;KUSUNOKI M;HATANAKA R;HAMANO K;NISHIKAWA H;HOSOI Y;HONTSU S
• 通讯作者: HONTSU S

Cytocompatibility of calcium phosphate coatings deposited by an ArF pulsed laser

ArF 脉冲激光沉积的磷酸钙涂层的细胞相容性

• 期刊: J Mater Sic Mater in Med (impress)
• 作者: Y.HASHIMOTO;M.KAWASHIMA;R.HATANAKA;M.KUSUNOKI;H.NISHIKAWA;S.HONTSU;M.NAKAMURA
• 通讯作者: M.NAKAMURA