A study on analysis of oral microbiota of oral cancer patients and application to management perisurgical period

口腔癌患者口腔微生物群分析及其在围手术期管理中的应用研究

• 批准号: 22592221
• 负责人: OOBU Kazunari
• 金额: $ 2.83万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592221/
• 关键词: 口腔がん; 口腔細菌叢; 唾液; T-RFLP法; 放射線治療; 化学放射線療法; 口腔粘膜炎; 口腔癌; 臨床歯学; 術前放射線化学療法

The purpose of this study is to find oral microbiota peculiar to oral cancer patient and to investigate whether oral microbiota changes according to the cancer treatment by using the patient's saliva.An amount of oral microbiota was highest before starting cancer treatment, and lowest during chemoradiotherapy. Though the proportion of Neisseria species and Rothia species became higher under the chemoradiotherapy analyzed by using T-RFLP method, those increase of proportions were not significant statistically analyzed by using Ion PGM method.These findings suggest that the influence of chemoradiotherapy and surgery on microbiota was not significant, and changes of oral microbiota differ from patient to patient.

本研究的目的是寻找口腔癌患者特有的口腔微生物群，并通过患者的唾液来探讨口腔微生物群是否会随着癌症的治疗而发生变化。口腔微生物群的数量在开始癌症治疗前最高，在放化疗期间最低。T-RFLP法分析放化疗后奈瑟菌属（Neisseria）和罗氏菌属（Rothia）的比例升高，但离子PGM法分析二者比例升高无统计学意义。这些结果提示放化疗和手术对口腔微生物群的影响不显著，患者口腔微生物群的变化也存在差异。