Research for Finding the Receptor of Epstein-Barr Virus Infection in Oral Squamous Cell Carcinomas

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We reported high incidence of Epstein-Barr virus (EVB) infection on both primary lesion and cervical lymphnodes in oral squamous cell carcinoma (OCSS). In order to reveal a mechanism of EBV infection to OSCC cells, EBV positive Akata cell (given from department of virology, Hokkaido Univ.) and CD21 cDNA (given from Alabama Univ. USA) were prepared. Squamous cell carcinoma cell line expressing EBV receptor CD21 (SAS-CD21-2) was established. SAS cells were transfected with 1μg pLNX-CR2 which contained CD21 and neomycin resistant genes, and had previously coated with lipopolyamine. Selection of cells containing stably integrated DNA was accomplished in medium containing Geneticin for 2 weeks. SAS transfectants were analyzed by flow cytometry using the OKB7 antibody. After the 10th passage, CD21 molecule was detected at the cell surface by analyses of flow cytometry. Therefore, we concluded that this transfectant cell line was stable line. The features of the SAS-CD21-2 were a little smaller than the original SAS cells. Growth doubling time of culture cells was approximately 25 hours. These SAS-CD21-2 cells were easier infected than the parent SAS cells by EB virus with the cell-to-cell contact manner like a case of gastric carcinomas (reported in 47^<th> Annual Meeting of Japanese Society of Oral and Maxillofacial Surgeons. 1st Nov. 2002, Sapporo).