Bacterial influence to the etiology of temporomandibular joint disorders;a cloning and sequencing analysis

细菌对颞下颌关节疾病病因的影响；克隆和测序分析

• 批准号: 22592236
• 负责人: SEGAMI Natsuki
• 金额: $ 3万
• 依托单位: Kanazawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592236/
• 关键词: 顎関節症; 細菌感染; 滑液; 遺伝子解析

Universal bacterial primers were used to amplify 16S rRNA genes in 31 synovial fluid samples from 31 patients with TMD (TMD group). Subsequently, PCR amplicons were purified and cloned. Partial 16S rRNA sequences of the cloned insert were used to determine the species identities or closest relative by comparison with known sequences using GeneBank. In results, 20 of 31 samples (64.5%) from patients in the TMD group were identified with the presence of bacterial DNA by PCR. The 5 control samples from the nonTMD group were all negative in this study. In conclusion, a wide variety of bacteria, including some not previously reported associated with TMD, wereidentified in the synovial fluids from patients with TMD.

应用细菌通用引物对31例TMD患者（TMD组）的31份关节液标本进行16SrRNA基因扩增。随后，纯化并克隆PCR扩增子。将克隆插入片段的部分16S rRNA序列与GeneBank中已知序列进行比较，以确定物种身份或最近的亲缘关系。结果，TMD组患者的31份样本中有20份（64.5%）通过PCR鉴定出细菌DNA的存在。非TMD组5例对照均为阴性。总之，在TMD患者的滑液中发现了多种细菌，包括一些以前未报道的与TMD相关的细菌。

项目成果

Increased Levels of Soluble Cytokine Receptors in the Synovial Fluid of Temporomandibular Joint Disorders in Relation to Joint Effusion on Magnetic Resonance Images

• DOI: 10.1016/j.joms.2009.10.027
• 发表时间: 2010-05-01
• 期刊: JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
• 影响因子: 1.9
• 作者: Kaneyama, Keiseki;Segami, Natsuki;Demura, Noboru
• 通讯作者: Demura, Noboru

Surgical reduction for TMJ dislocation via simplified direct approach techniaque-A survey 40 patients

通过简化直接入路技术进行颞下颌关节脱位手术复位-A 调查 40 名患者

• 发表时间: 2012
• 作者: 金山景錫;八田稔久;吉村弘;青木秀哲;小野徹;瀬上夏樹;金山景錫;kaneyama K.Segami N.Nishiura R.Yoshimura H.;Kaneyama K;Segami N.;Kaneyama K.Segami N.Yoshimura H.Honjo M.Demura N.;Segami N;Kaneyama K.;Segami N.;N.Segami
• 通讯作者: N.Segami

よくわかる歯科医学・口腔ケア、顎関節脱臼

通俗易懂的牙科/口腔护理、颞下颌关节脱位

• 发表时间: 2011
• 作者: 瀬上夏樹;他;瀬上夏樹
• 通讯作者: 瀬上夏樹

High condylectomy and/or eminectomy under local anesthesia for medically compromised patients with habitual dislocation of the temporomandibular joint.

在局麻下对患有颞下颌关节习惯性脱位的医学上受损的患者进行高位髁切除术和/或股骨头切除术。

• 发表时间: 2010
• 期刊: European Association of Cranio-Maxillofacial surgery
• 作者: 金山景錫;八田稔久;吉村弘;青木秀哲;小野徹;瀬上夏樹;金山景錫;kaneyama K.Segami N.Nishiura R.Yoshimura H.;Kaneyama K;Segami N.;Kaneyama K.Segami N.Yoshimura H.Honjo M.Demura N.;Segami N
• 通讯作者: Segami N

Surgical reduction for TMJ dislocation via simplified direct approach techniaque-A survey 40 patients.

通过简化直接入路技术对 TMJ 脱位进行手术复位 - A 调查 40 名患者。

• 发表时间: 2012
• 作者: 金山景錫;八田稔久;吉村弘;青木秀哲;小野徹;瀬上夏樹;金山景錫;kaneyama K.Segami N.Nishiura R.Yoshimura H.;Kaneyama K;Segami N.;Kaneyama K.Segami N.Yoshimura H.Honjo M.Demura N.;Segami N;Kaneyama K.;Segami N.
• 通讯作者: Segami N.