Innovative method of combined hepatic tissue regeneration by the connection of bile canaliculi and bile ducts

胆小管与胆管连接联合肝组织再生的创新方法

• 批准号: 22650106
• 负责人: SUDO Ryo
• 金额: $ 2万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650106/
• 关键词: 人工臓器工学; 再生医工学; 胆管上皮細胞

The purpose of this study is to reconstruct a combined hepatic tissue by controlling oxygen concentration and elasticity in cellular microenvironments. Specifically, we aimed to combine bile canaliculi, which are formed by hepatocytes, and bile ducts, which are formed by biliary epithelial cells. Oxygen concentration was controlled using an oxygen permeable silicone membrane while elasticity was controlled by changing the elasticity of collagen gel that is a cellular scaffold. We found that oxygen concentration around cells is an important regulatory factor for combining bile ducts and bile canaliculi.

本研究的目的是通过控制细胞微环境中的氧浓度和弹性来重建联合肝组织。具体而言，我们的目标是将由肝细胞形成的联合收割机胆小管和由胆管上皮细胞形成的胆管结合。使用透氧硅膜控制氧浓度，同时通过改变作为细胞支架的胶原凝胶的弹性来控制弹性。我们发现细胞周围的氧浓度是胆管和胆小管结合的重要调节因素。

项目成果

生体吸収性薄膜による胆管上皮細胞の管腔構造形成

利用生物可吸收薄膜形成胆管上皮细胞的管腔结构

• 发表时间: 2010
• 作者: 松尾薫;他
• 通讯作者: 他

A bioengieering approach to liver tissue engineering

肝组织工程的生物工程方法

• 发表时间: 2010
• 作者: 谷下一夫;須藤亮;Ryo Sudo
• 通讯作者: Ryo Sudo

Microfluidic Hydrostatic Deposition Patterning for investigatinghepatocyte-biliary epithelial cell interactions

用于研究肝细胞-胆管上皮细胞相互作用的微流体静水沉积模式

• 发表时间: 2011
• 作者: Yuyang Lee;Ryo Sudo;Tomoya Komatsu;Norihisa Miki;Toshihiro Mitaka;Mariko Ikeda;Kazuo Tanishita;Yuyang Lee
• 通讯作者: Yuyang Lee

Microfluidic hydrostatic deposition patterning for a confined hepatocyte-biliary epithelial cell co-culture system

有限肝细胞-胆管上皮细胞共培养系统的微流体静水沉积图案

• 发表时间: 2011
• 作者: Sudo;R.;Tanishita;K.;et al
• 通讯作者: et al

Effect of Extracellular Matrix Stiffness on Ductular Formation of Biliary Epithelial Cells

细胞外基质硬度对胆管上皮细胞导管形成的影响

• 发表时间: 2010
• 作者: Komatsu N;et al
• 通讯作者: et al