Innovative method of combined hepatic tissue regeneration by the connection of bile canaliculi and bile ducts

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The purpose of this study is to reconstruct a combined hepatic tissue by controlling oxygen concentration and elasticity in cellular microenvironments. Specifically, we aimed to combine bile canaliculi, which are formed by hepatocytes, and bile ducts, which are formed by biliary epithelial cells. Oxygen concentration was controlled using an oxygen permeable silicone membrane while elasticity was controlled by changing the elasticity of collagen gel that is a cellular scaffold. We found that oxygen concentration around cells is an important regulatory factor for combining bile ducts and bile canaliculi.