Inhibitory effects of exercise on progression of obesity-related liver diseases and development of glyco-biomarkers to scale the pathophysiology of the liver diseases

在肥胖相关的肝病，即非酒精性脂肪性肝病的治疗中，增加骨骼肌对葡萄糖的利用，从而改善胰岛素抵抗是重要的。因此，在患有非酒精性脂肪肝的受试者中，通过适当的运动来维持骨骼肌量也是很重要的。在评估运动对非酒精性脂肪性肝病肝脏病理生理的影响时，我们需要探索能够从脂肪变性、炎症和纤维化等方面反映肝脏病理生理状态的新的生物标志物，并建立这些生物标志物的非侵入性测量系统。在本研究项目中，利用参加减肥疗程的中年肥胖男性的血清样本，我们探索了新的生物标志物，可以衡量运动对肝脏病理生理的改善。我们测定了血清样品中N-乙酰氨基葡萄糖基转移酶V(GnT-V)的活性作为糖生物标志物。与限食相比，运动增加了能量消耗，维持了瘦体重，并降低了血清铁蛋白和氧化应激标志物TBARS的水平。综上所述，血清铁蛋白和TBARS水平很可能是衡量运动对肥胖男性肝脏病理生理影响的有用生物标志物。正在探索新的乙二醇类生物标记物。

In management of obesity-related liver diseases, that is, non-alcoholic fatty liver diseases, it is important to increase a utilization of glucose in the skeletal muscles and thereby to improve the insulin-resistance. Therefore, in subjects with non-alcoholic fatty liver diseases, it is also important to maintain the volume of skeletal muscles by appropriate exercise. In the assessment of effects of exercise on liver pathophysiology of non-alcoholic fatty liver diseases, we need to explore novel biomarkers that can reflect the status of liver pathophysiology in terms of steatosis, inflammation, and fibrosis, and to develop a non-invasive measurement system of these biomarkers. In this research project, using the serum samples of middle-aged obese men who joined a weight-losing course, we explored novel biomarkers that can scale the improvement of liver pathophysiology induced by exercise. We measured an enzyme activity of N-acetylglucosaminyltransferase V (GnT-V) in the serum samples as a glyco-biomaker. Exercise increased the energy consumption, maintained the lean body mass, and decreased the serum levels of ferritin and TBARS, an oxidative stress marker, in comparison with diet restriction. Taken together, it is likely that serum levels of ferritin and TBARS are useful biomarkers to scale the effects of exercise on liver pathophysiology in obese men. Exploration of novel glycol-biomarkers is being performed.