Improvement of metabolic and exercise function of skeletal muscles through activation of transcription factor, and prevention of obesity-related liver disease

每组小鼠均进行最大运动耐量试验。结果表明，Nrf2KO小鼠的跑步距离明显短于野生型小鼠。在Nrf2被有效激活剂Sulforaphan激活的野生型小鼠中，观察到这一距离变得更长。在野生型小鼠中观察到运动对高糖高脂饮食小鼠肝脏脂肪变性的改善作用，而在Nrf2KO小鼠中未观察到运动对小鼠肝脏脂肪变性的改善作用。因此，我们从小鼠肌肉组织中培养出了心肌细胞，并研究了运动训练对细胞内肌细胞因子产生和分泌的影响。电刺激后，Nrf2在心肌细胞中被激活，IL-6的产生明显增加。Nrf2在心肌细胞中的表达可能是影响运动诱导的肌肉细胞因子产生和分泌的一个重要因素。

Each mouse group was subjected to the maximum exercise tolerance test. The results showed that the running distance was significantly shorter in Nrf2KO mice than in wild-type mice. The distance was observed to become longer in the wild-type mice with Nrf2 activation by sulforaphan, a potent Nrf2 activator. The ameliorating effect of exercise on hepatic steatosis of mice fed high sucrose and fat diet was observed in the wild-type mice but not in the Nrf2KO mice. Therefore, we developed cultured myocytes from mouse muscle tissues and investigated the effect of exercise training on myokine production and secretion in the cells. Upon electrical stimulation, Nrf2 was observed to be activated in the myocytes and the production of IL-6, a myokine was markedly increased in the cells. It is likely that Nrf2 expression in myocytes is an important factor influential on exercised-induced myokine production and secretion in the muscles.