Inflammation-Associated Lipid Mediators in Cholestatic Hepatobiliary Diseases and Effect of PAF-AH

胆汁成分穿过小管膜的转运是胆汁形成的限速步骤。由于长期的胆道梗阻和胆道感染相关的胆管炎并发症通过炎症相关的氧化应激对肝脏分泌功能有很大的影响，因此应该关注肝胆疾病患者胆汁淤积性肝脏中小管膜转运蛋白的表达水平。我们测定了肝内胆管结石患者和接受术前胆道引流的梗阻性胆汁淤积患者肝脏中胆红素结合物和胆汁酸小管转运蛋白的分子和免疫组化表达。此外，它们的表达水平与胆汁分泌受损相关。本研究得出结论，在肝胆疾病的肝脏中，小管转运蛋白的表达改变可能与胆汁形成和分泌受损有关，可能是通过炎症相关的氧化应激对肝脏造成的。

The transport of biliary constituents across the canalicular membrane is the rate-limiting step in bile formation. Since long-standing biliary obstruction and complication of cholangitis associated with biliary infection have a large influence upon the hepatic secretory function through inflammation-related oxidative stress on the liver, interest should be focused on the expression levels of canalicular membrane transporter proteins in the cholestatic liver of patients with hepatobiliary diseases. We determined the molecular and immunohistochemical expression of canalicular transporter proteins, for bilirubin conjugate and bile acid,in the liver of patients with hepatolithiasis and in those of the patients with obstructive cholestasis who had undergone preoperative biliary drainage. Furthermore, their expression levels were correlated with the impairment of biliary secretion.This study concludes that in the liver of hepatobiliary diseases, the altered expression of the canalicular transporters may be associated with the impairment of bile formation and secretion, propably through inflammation-related oxidative stress on the liver.