Development of a novel cancer immunotherapy using the artificial proteins consisted in the functional peptide motifs.

我们通过组合组装分别与卵清蛋白(OVA)的MHC I类表位和MHC II类表位相关的四个肽基序、α螺旋基序和随机化的肽序列，构建了一个人工蛋白库。我们已经产生了两种人工蛋白，F37A和F182A，它们能够在不使用任何佐剂的情况下诱导OVA特异性细胞免疫。这些外源蛋白通过抗原提呈细胞中的交叉提呈途径呈递OVA特异性MHC I类表位。我们的研究表明，基序程序化的人工蛋白可能能够诱导细胞免疫，有助于癌症的免疫治疗。

We have constructed an artificial protein library by combinatorially assembling four peptide motifs associated with the MHC class I epitope and MHC class II epitope of OVA(Ovalbumin), alpha-helical motif and randomized peptide sequence, respectively. We have generated two artificial proteins, F37A and F182A, which have the ability to induce OVA-specific cellular immunity without using any adjuvant. These exogenous proteins presented OVA-specific MHC class I epitope through the cross-presentation pathway in the antigen presenting cells. Our studies have demonstrated that the motif-programmed artificial proteins might be able to induce cellular immunity contribute to cancer immunotherapy.