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Molecular mechanisms of regulation of cell division by Myb transcription factors in plants.

植物中 Myb 转录因子调节细胞分裂的分子机制。

基本信息

批准号：
17570032
负责人：
ITO Masaki
金额：
$ 2.3万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570032/
关键词：
cell cycle plant cell cell proliferation transcriptional regulation Arabidopsis thaliana cytokinesis

项目摘要

Myb transcription factors with three repeats in the Myb domain (3R-Myb) are encoded by a relatively small subfamily in the large Myb gene family in plants. We have previously shown that this type of Myb proteins have important roles in regulation of G2/M-phase genes in tobacco. The 3R-Myb proteins in plants can be divided into 3 classes (A-type, B-type and C-type 3R-Myb) based on their primary sequences. The aim of this research was to elucidate the molecular mechanisms of transcriptional regulation by 3R-Myb and to identify novel factors that regulate activities of 3R-Myb proteins.1. We showed that C-type 3R-Myb proteins act as transcriptional repressors on CYCB1 promoter in tobacco cells.2. The transactivation potential of NtmybA2, a tobacco A-type 3R-Myb protein, is repressed by a regulatory domain located at its C terminus. Deletion of this domain dramatically enhanced its transactivation activity. Our microarray expriments revealed that overexpression of C-terminally deleted version of NtmybA2 resulted in selective upregulation of many G2/M genes that contain MSA elements. The results allowed a genome-wide identification of potential target genes of transcriptianl regulation by NtmybA2 in tobacco cells.3. In another approach, we aimed to identify the mutation that enhances the cytokinesis defect caused by T-DNA insertion mutation of myb3r4, an A-type 3R-Myb gene in Arabidopsis thaliana. We found that one such mutation is present in genome of the wild-type Lansberg. We could map its locus within a 400-kb region located in upper arm of chromosome 3. Further mapping of this locus and complementation experiments would allow us to identify the gene that are responsible for enhancing the cytokinesis defect in myb3r4 background.

Myb转录因子（3R-Myb）是由植物中Myb基因家族中一个相对较小的亚家族编码的。我们以前已经表明，这种类型的Myb蛋白在烟草G2/M期基因的调控中具有重要作用。植物中的3R-Myb蛋白根据其一级序列可分为3类（A型、B型和C型）。本研究的目的是阐明3R-Myb转录调控的分子机制，并鉴定新的调控3R-Myb蛋白活性的因子.我们发现C型3R-Myb蛋白在烟草细胞中充当CYCB 1启动子的转录抑制子。2.烟草A型3R-Myb蛋白NtmybA 2的反式激活潜力被位于其C末端的调节结构域抑制。该结构域的缺失显著增强了其反式激活活性。我们的微阵列实验显示，NtmybA 2的C端缺失版本的过表达导致许多含有MSA元件的G2/M基因的选择性上调。该结果允许在烟草细胞中鉴定NtmybA 2转录调控的潜在靶基因.在另一种方法中，我们的目的是确定突变，增强所造成的胞质分裂缺陷的T-DNA插入突变的myb 3r 4，A型3R-Myb基因在拟南芥。我们发现一个这样的突变存在于野生型Lansberg的基因组中。我们可以将其定位在位于3号染色体上臂的400 kb区域内。进一步定位该基因座和互补实验将使我们能够鉴定在myb 3r 4背景下负责增强胞质分裂缺陷的基因。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Transcriptional activation of tobacco E2F is repressed by co-transfection with the retinobalastoma-related protein : cyclin D expression overcomes this repressor activity.

烟草 E2F 的转录激活受到视网膜母细胞瘤相关蛋白共转染的抑制：细胞周期蛋白 D 的表达克服了这种抑制活性。

DOI：
发表时间：
2005
期刊：
Plant Mol Biol. 57
影响因子：
0
作者：
Uemukai;K.
通讯作者：
K.

R1R2R3-Myb proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana

DOI：
10.1242/dev.02801
发表时间：
2007-03-15
期刊：
DEVELOPMENT
影响因子：
4.6
作者：
Haga, Nozomi;Kato, Kiichi;Ito, Masaki
通讯作者：
Ito, Masaki

Transcriptional activation of tobacco E2F is repressed by co-transfection with the retinoblastoma-related protein: cyclin D expression overcomes this repressor activity

DOI：
10.1007/s11103-004-6601-x
发表时间：
2004
期刊：
Plant Molecular Biology
影响因子：
5.1
作者：
Kenji Uemukai;H. Iwakawa;S. Kosugi;Sarah de Uemukai;K. Kato;E. Kondorosi;J. Murray;Masaki Ito;A. Shinmyō;M. Sekine
通讯作者：
Kenji Uemukai;H. Iwakawa;S. Kosugi;Sarah de Uemukai;K. Kato;E. Kondorosi;J. Murray;Masaki Ito;A. Shinmyō;M. Sekine

Conservation and diversification of three-repeat Myb transcription factors in plants