Study of mechanosensing in the spindle using a 3D force microscope

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As a cell changes its shape such as cell division, one of the cytoskeletons, a microtubule, is subject to mechanical stress and responds to mechanical stimulation. These responses may regulate biochemical and mechanical reactions in cell. One way to understand its regulated system is to apply mechanical stress to a microtubule in an in vitro assay and then to quantify motility of molecular motors(kinesins and dyneins) interacted with the microtubule. We constructed microscope systems, which are able to apply mechanical force into the microtubule in 3D dimensions. Using this system, we are examining if a microtubule is mechano-sensor.