Investigations of molecular mechanism of Eg5 and inhibitory mechanism of Eg5 inhibitors.

我们已经发现，微管在表面上滑动，附着在单个驱动蛋白-5头上，这对于在细胞分裂时构建有丝分裂纺锤体至关重要，它们绕着它们的长轴以固定的速度旋转。通过对附着在滑动微管上的量子点进行三维纳米分辨率光学跟踪，实现了对微管螺旋运动的显著观察。新方法只使用一个光学元件（棱镜），而不需要对光学装置的其他方面进行任何修改。

We have discovered that microtubule sliding over surface-attached to single kinesin-5 heads, which is essential for building a mitotic spindle at cell division, rotate at a fixed rate around their long axis. This remarkable observation of a corkscrewing motion of the microtubule was achieved by three-dimensional nanometer resolution optical tracking of a quantum dot attached to the sliding microtubule. The new method uses only one optical component (a prism) without any modification of other aspect of the optical setups.