Investigations of molecular mechanism of Eg5 and inhibitory mechanism of Eg5 inhibitors.

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We have discovered that microtubule sliding over surface-attached to single kinesin-5 heads, which is essential for building a mitotic spindle at cell division, rotate at a fixed rate around their long axis. This remarkable observation of a corkscrewing motion of the microtubule was achieved by three-dimensional nanometer resolution optical tracking of a quantum dot attached to the sliding microtubule. The new method uses only one optical component (a prism) without any modification of other aspect of the optical setups.